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Journal of Histochemistry and Cytochemistry, Vol. 50, 1023-1030, August 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

Region-specific Antibodies to Chromogranin B Display Various Immunostaining Patterns in Human Endocrine Pancreas

Guida Maria Portela–Gomesa,c and Mats Stridsbergb
a Department of Genetics and Pathology, Unit of Pathology, University of Lisbon, Lisbon, Portugal
b Department of Medical Sciences, Clinical Chemistry, University of Lisbon, Lisbon, Portugal
c University Hospital, Uppsala, Sweden, and the Centres of Gastroenterology and of Nutrition, University of Lisbon, Lisbon, Portugal

Correspondence to: Guida Maria Portela–Gomes, Dept. of Genetics and Pathology, Unit of Pathology, University Hospital, 75185 Uppsala, Sweden. E-mail: portela_gomes@yahoo.com

Chromogranin (Cg) B is an acidic glycoprotein present in neuroendocrine tissue. The sequence shows several dibasic amino acid positions susceptible to proteolytic cleavage. The purpose of this study was to elucidate the expression of CgB epitopes in the human endocrine pancreas. Tissue sections of six human pancreata were immunostained with 16 different region-specific antibodies to the CgB molecule, using double immunofluorescence techniques. The CgB epitope pattern varied in the four major islet cell types. B (insulin)-cells expressed immunoreactivity to all region-specific antibodies. The antibodies to the N-terminal and mid-portions of CgB showed moderate immunoreactivity, the C-terminal antibodies weak. A (glucagon)-cells were reactive only to the N-terminal and mid-portion antibodies but, after microwave pretreatment, to all antibodies, whereas D (somatostatin)-cells expressed only the sequence CgB 244–255 and a subpopulation CgB 580–595. PP (pancreatic polypeptide) cells were immunostained with antibodies between CgB 1–417 and a few with CgB 580–593. The fragment CgB 244–255 was expressed in all four cell types. The cause of these differences may be cell-specific cleavage or masking of the molecule, but varying translation of CgB mRNA is also possible. The extent to which these epitopes reflect fragments having biological functions remains to be evaluated.

(J Histochem Cytochem 50:1023–1030, 2002)

Key Words: chromogranin B, chromogranin B fragments, immunohistochemistry, pancreas, human


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