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Journal of Histochemistry and Cytochemistry, Vol. 50, 1059-1065, August 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

Distribution of the Transcription Factors Sox9, AP-2, and [Delta]EF1 in Adult Murine Articular and Meniscal Cartilage and Growth Plate

Sherri R. Daviesa, Shinji Sakanoa, Yong Zhua, and Linda J. Sandella
a Washington University School of Medicine at Barnes–Jewish Hospital, Department of Orthopaedic Surgery, St Louis, Missouri

Correspondence to: Linda J. Sandell, Washington U. School of Medicine, Dept. of Orthopaedic Surgery, Mail Stop 90-34-674, 216 South Kingshighway, St Louis, MO 63110. E-mail: sandelll@msnotes.wustl.edu

The control of extracellular matrix (ECM) production is important for the development, maintenance, and repair of cartilage tissues. Matrix molecule synthesis is generally regulated by the rate of gene transcription determined by DNA transcription factors. We have shown that transcription factors Sox9, AP-2, and [delta]EF1 are able to alter the rate of CD-RAP transcription in vitro: Sox9 upregulates, AP-2 exhibits biphasic effects, and [delta]EF1 represses expression of the CD-RAP gene. To correlate these in vitro activities in vivo, transcription factors were co-immunolocalized with ECM proteins in three different cartilage tissues in which the rates of biosynthesis are quite different: articular, meniscal, and growth plate. Immunoreactivities of type II collagen and CD-RAP were higher in growth plate than in either the articular or meniscal cartilages and correlated positively with Sox9 protein. Sox9 staining decreased with hypertrophy and was low in articular and meniscal cartilages. In contrast, AP-2 and [delta]EF1 were low in proliferating chondrocytes but high in lower growth plate, articular, and meniscal cartilages. This increase was also accompanied by intense nuclear staining. These immunohistochemical results are the first to localize both [delta]EF1 and AP-2 to adult articular, meniscal, and growth plate cartilages and provide in vivo correlation of previous molecular biological studies.

(J Histochem Cytochem 50:1059–1065, 2002)

Key Words: type II collagen, CD-RAP, AP-2, Sox9, [delta]EF1, cartilage


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[Abstract] [Full Text] [PDF]



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