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Journal of Histochemistry and Cytochemistry, Vol. 50, 1131-1134, August 2002, Copyright © 2002, The Histochemical Society, Inc.

BRIEF REPORT

Monoclonal Antibody CD133-2 (AC141) Against Hematopoietic Stem Cell Antigen CD133 Shows Crossreactivity with Cytokeratin 18

Andy J.G. Pötgensa, Ulrike Schmitza, Peter Kaufmanna, and Hans-Georg Franka
a Department of Anatomy, University Hospital Aachen, Aachen, Germany

Correspondence to: Andy J.G. Pötgens, Dept. of Anatomy, University Hospital Aachen, Wendlingweg 2, D-52057 Aachen, Germany. E-mail: apotgens@ukaachen.de

CD133 is an antigen expressed on hematopoietic progenitor cells and on some epithelial cells. We previously reported that a commercially available antibody against CD133, CD133-2/AC141, also reacted with an intracellular protein in placental trophoblasts. Here we show by 2D electrophoresis and mass spectroscopy that this reactivity is with cytokeratin 18, a cytokeratin present in most simple epithelia. Immunohistochemistry (IHC) with CD133-2/AC141 on a trophoblast cell line displayed a staining pattern typical for the cytoskeleton. Cryostat sections of stratified epithelia lacking cytokeratin 18 did not react with CD133-2/AC141. In conclusion, care must be taken not to misinterpret staining patterns using CD133-2/AC141 in IHC. (J Histochem Cytochem 50:1131–1134, 2002)

Key Words: CD133, AC141, PROML1, cytokeratin 18, hematopoietic stem cells, epithelial cells, human


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