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Journal of Histochemistry and Cytochemistry, Vol. 50, 999-1003, August 2002, Copyright © 2002, The Histochemical Society, Inc.

RAPID COMMUNICATION

An Antigen Expressed During Plant Vascular Development Crossreacts with Antibodies Towards KLH (Keyhole Limpet Hemocyanin)

Anna-Stina Höglunda, Alan M. Jonesb, and Lars-Göran Josefssona
a Department of Plant Biology, Swedish University of Agricultural Sciences, Uppsala, Sweden
b Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina

Correspondence to: Lars-Göran Josefsson, Dept. of Plant Biology, Swedish U. of Agricultural Sciences, Box 7080, SE-750 07 Uppsala, Sweden. E-mail: Lars-Goran.Josefsson@vbiol.slu.se

An antigen present in plant vascular tissue crossreacts with antibodies towards keyhole limpet hemocyanin (KLH). The antigen is present in xylem and vascular cambium, as evidenced by immunocytochemical staining of plant sections. This cell type assignment was confirmed by staining of mesophyll cell cultures from Zinnia elegans L. undergoing tracheary cell differentiation. The strongest staining both in sections and cell cultures occured in cells and tissues during early stages of differentiation. Although the anti-KLH antibodies can easily be removed by affinity purification, our findings suggest that a certain caution is needed when KLH is used as an immunological carrier for studies in plants. (J Histochem Cytochem 50:999–1003, 2002)

Key Words: plant vascular development, tracheary cell culture, xylem, programmed cell death, keyhole limpet hemocyanin, immunological carrier, immunocytochemical staining, crossreactivity


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[Abstract] [Full Text] [PDF]



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