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Journal of Histochemistry and Cytochemistry, Vol. 50, 1179-1186, September 2002, Copyright © 2002, The Histochemical Society, Inc.

ARTICLE

Distribution of Sialoglycoconjugates in the Rat Cerebellum and Its Change with Aging

Tasuku Sasakia, Yoshihiro Akimotob, Yuji Satoa, Hayato Kawakamib, Hiroshi Hiranob,c, and Tamao Endoa
a Department of Glycobiology, Tokyo Metropolitan Institute of Gerontology
b Department of Anatomy, Kyorin University School of Medicine, Tokyo, Japan
c Nittai Jusei Medical College for Judo Therapeutics, Tokyo, Japan

Correspondence to: Tamao Endo, Dept. of Glycobiology, Tokyo Metropolitan Inst. of Gerontology, 35-2 Sakaecho, Itabashi-ku, Tokyo 173-0015, Japan. E-mail: endo@tmig.or.jp

We examined the distribution of sialoglycoconjugates in the cerebellum of 9-week-old and 30-month-old rats using light microscopy and electron microscopy in combination with two lectins, Maackia amurensis lectin (MAL) for Sia{alpha}2–3Gal and Sambucus sieboldiana agglutinin (SSA) for Sia{alpha}2–6Gal. Each lectin showed characteristic staining patterns. In young adult rats, MAL stained a strongly granular layer, a weakly molecular layer, and the medullary lamina, while SSA more strongly stained the medullary lamina than the molecular and granular layers. After aging, different staining patterns were obtained. Intense SSA reactivity was observed in the granular layer and intense MAL reactivity was observed in the medullary lamina of the aged groups. The reactivity of Purkinje cells with MAL was downregulated in the aged rats. These results indicated that Sia{alpha}2–3Gal and Sia{alpha}2–6Gal were expressed in distinct regions of the rat cerebellum and that their expression patterns changed in the aged brain. (J Histochem Cytochem 50:1179–1186, 2002)

Key Words: sialoglycoconjugate, cerebellum, aging, granular layer, Purkinje cell


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