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PERSPECTIVES |
The Antigen–Antibody Reaction in Immunohistochemistry
Claudio Monteroaa Seville, Spain
Correspondence to: Claudio Montero, Plaza Rafael Salgado 30-3°D, 41013 Sevilla, Spain. E-mail: montclau@supercable.es
The problems of major concern in immunohistochemical practice are discussed in the following order: (a) the mechanism of the Ag–Ab reaction in fixed tissue as opposed to the in vitro reaction; (b) the chemistry of fixation and its influence on the final result of the immunohistochemical reaction; (c) the various procedures used for antigen retrieval in formaldehyde-fixed tissue; and (d) the consideration of the possible mechanism underlying heat-induced antigen retrieval. Suggestions for further work to attempt a clarification of the mechanism involved in the Ag–Ab reaction in immunohistochemistry resorting to existing histochemical methods for the demonstration of protein side groups are presented, together with some examples already published.
(J Histochem Cytochem 51:1–4, 2003)
Key Words: antigen–antibody reaction, antigen retrieval, fixation, immunohistochemistry
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