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Journal of Histochemistry and Cytochemistry, Vol. 51, 113-119, January 2003, Copyright © 2003, The Histochemical Society, Inc.

ARTICLE

Myosin Heavy Chain Composition in Human Masticatory Muscles by Immunohistochemistry and Gel Electrophoresis

J.A.M. Korfage and T.M.G.J Van Eijden
a Department of Functional Anatomy, Academic Centre for Dentistry Amsterdam, Amsterdam, The Netherlands

Correspondence to: T.M.G.J Van Eijden, Dept. of Functional Anatomy, Academic Centre for Dentistry Amsterdam (ACTA), Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands. E-mail: j.a.korfage@amc.uva.nl

In this study we compared the immunohistochemically quantified fiber type area with the myosin heavy chain (MyHC) contents of a bundle of fibers from a human masticatory muscle. The total cross-sectional areas were determined immunohistochemically for the three major fiber types (I, IIA, and IIX) in bundles of fibers (n=42) taken from the anterior and posterior belly of the human digastric muscle (n=7). The relative MyHC contents of the same fiber bundles were determined electrophoretically (MyHC-I, -IIA, and -IIX; anterior, 32%, 35%, and 33%; posterior, 39%, 42%, and 19%) and compared with the immunohistochemical data (MyHC-I, -IIA, and -IIX; anterior, 32%, 31%, and 37%; posterior, 39%, 45%, and 15%). No significant differences were seen in the mean fiber type distribution between the two techniques; the correlation coefficient ranged from 0.71 to 0.96. The correlation coefficient was higher for MyHC type I and MyHC type IIX than for MyHC type IIA. The MyHC contents of single fibers taken from the posterior belly indicated that many fibers in this belly co-express MyHC-IIA and MyHC-IIX. Despite the presence of these hybrid fibers, the correspondence between both methods was relatively large.

(J Histochem Cytochem 51:113–119, 2003)

Key Words: myosin, SDS-PAGE, immunohistochemistry, digastric muscle


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