Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Piludu, M.
Right arrow Articles by Hand, A. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Piludu, M.
Right arrow Articles by Hand, A. R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 51, 69-79, January 2003, Copyright © 2003, The Histochemical Society, Inc.

ARTICLE

Electron Microscopic Immunogold Localization of Salivary Mucins MG1 and MG2 in Human Submandibular and Sublingual Glands

Marco Piludua, Sean A. Raymentb, Bing Liub, Gwynneth D. Offnerc,d, Frank G. Oppenheimb,c, Robert F. Troxlerb,c, and Arthur R. Hande
a Departimento di Citomorfologia, Universita Degli Studi di Cagliari, Cagliari, Italy
b Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, School of Medicine, Boston, Massachusetts
c Departments of Biochemistry, Boston University, School of Medicine, Boston, Massachusetts
d Medicine, Boston University, School of Medicine, Boston, Massachusetts
e Department of Pediatric Dentistry, School of Dental Medicine, University of Connecticut, Farmington, Connecticut

Correspondence to: Arthur R. Hand, Dept. of Pediatric Dentistry, U. of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030-1610. E-mail: hand@nso1.uchc.edu

The human salivary mucins MG1 and MG2 are well characterized biochemically and functionally. However, there is disagreement regarding their cellular and glandular sources. The aim of this study was to define the localization and distribution of these two mucins in human salivary glands using a postembedding immunogold labeling method. Normal salivary glands obtained at surgery were fixed in 3% paraformaldehyde–0.1% glutaraldehyde and embedded in Lowicryl K4M or LR Gold resin. Thin sections were labeled with rabbit antibodies to MG1 or to an N-terminal synthetic peptide of MG2, followed by gold-labeled goat anti-rabbit IgG. The granules of all mucous cells of the submandibular and sublingual glands were intensely reactive with anti-MG1. No reaction was detected in serous cells. With anti-MG2, the granules of both mucous and serous cells showed reactivity. The labeling was variable in both cell types, with mucous cells exhibiting a stronger reaction in some glands and serous cells in others. In serous granules, the electron-lucent regions were more reactive than the dense cores. Intercalated duct cells near the acini displayed both MG1 and MG2 reactivity in their apical granules. In addition, the basal and lateral membranes of intercalated duct cells were labeled with anti-MG2. These results confirm those of earlier studies on MG1 localization in mucous cells and suggest that MG2 is produced by both mucous and serous cells. They also indicate differences in protein expression patterns among salivary serous cells. (J Histochem Cytochem 51:69–79, 2003)

Key Words: salivary glands, mucous cells, serous cells, intercalated ducts, immunohistochemistry/ postembedding, immunogold labeling


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
M. C. Rose and J. A. Voynow
Respiratory Tract Mucin Genes and Mucin Glycoproteins in Health and Disease
Physiol Rev, January 1, 2006; 86(1): 245 - 278.
[Abstract] [Full Text] [PDF]

Home page
 JDRHome page
S. Ruhl, S.A. Rayment, G. Schmalz, K.-A. Hiller, and R.F. Troxler
Proteins in Whole Saliva during the First Year of Infancy
Journal of Dental Research, January 1, 2005; 84(1): 29 - 34.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
M. Ahmad, M. Piludu, F. G. Oppenheim, E. J. Helmerhorst, and A. R. Hand
Immunocytochemical Localization of Histatins in Human Salivary Glands
J. Histochem. Cytochem., March 1, 2004; 52(3): 361 - 370.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2003