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Journal of Histochemistry and Cytochemistry, Vol. 51, 81-87, January 2003, Copyright © 2003, The Histochemical Society, Inc.

ARTICLE

Expression of Endothelial Nitric Oxide Synthase in Ciliated Epithelia of Rats

Xinhua Zhana, Dechun Lia, and Roger A. Johnsa
a Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland

Correspondence to: Roger A. Johns, Dept. of Anesthesiology and Critical Care Medicine, Blalock 1415, Johns Hopkins University School of Medicine, 600 N. Wolfe Street, Baltimore, MD 21287-4965. E-mail: rajohns@jhmi.edu

Endothelial nitric oxide synthase (eNOS), originally found in the endothelium of vascular tissue, also exists in other cell types, including ciliated epithelia of airways. The eNOS is ultrastructurally localized to the basal body of the microtubules of the cilia, and nitric oxide (NO) stimulates ciliary beat frequency (CBF). We examined whether the expression of eNOS is present in ciliated cells of other organs. Western blotting analysis revealed that eNOS was expressed in the rat cerebrum, lung, trachea, testis, and oviduct. Immunohistochemical staining showed that eNOS was localized in the ciliated epithelia of airways, oviduct, testis, and ependymal cells of brain in addition to the endothelium and smooth muscle of the vasculature. To confirm the activation of eNOS in the ciliated epithelia, we examined the effect of L-arginine (L-Arg), the substrate of NOS, on the production of nitrite and nitrate (NOx) in the cultured explants of rat trachea. L-Arg (100 µM) increased NOx levels significantly (p<0.05). In explants exposed to inhibitors of NOS, the effect of L-Arg on the production of NOx was blocked. These findings suggest that epithelial NO plays an important role in signal transduction associated with ciliary functions.

(J Histochem Cytochem 51:81–87, 2003)

Key Words: endothelial nitric oxide, synthase, ciliated epithelium, oviduct, testis, airway, ependyma


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