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Journal of Histochemistry and Cytochemistry, Vol. 51, 1317-1329, October 2003, Copyright © 2003, The Histochemical Society, Inc.

ARTICLE

Androgen Regulation of SMR2 Gene Expression in Rat Submandibular Gland: Evidence for a Graded but not a Binary Response

Jean-François Huaulméa, Yves Courtya, François Rougeona, and Isabelle Rosinski–Chupina
a Unité de Génétique et Biochimie du Développement, URA CNRS 1960, Département d'Immunologie, Institut Pasteur, Paris, France

Correspondence to: Isabelle Rosinski–Chupin, Unité de Biochimie et de Biologie Moléculaire des Insectes, Département Ecosystèmes et Epidémiologie des Maladies Infectieuses, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris, cedex 15, France. E-mail: ichupin@pasteur.fr

Expression of SMR2, a member of the gene family encoding salivary glutamine/glutamic acid-rich proteins, is regulated by androgens in rat submandibular gland acinar cells. To further characterize SMR2 regulation, we analyzed SMR2 expression during submandibular gland postnatal development and rat puberty at both a global and a single-cell level. Using in situ detection of mature and primary SMR2 transcripts, we show that SMR2 expression is heterogeneous among acinar cells. However, only one cell population with various amounts of mRNAs can be defined. The number of high-expressing cells increases in males during puberty and in females up to 6 weeks of age, suggesting that some factor in addition to acinar differentiation might be important for SMR2 expression in female rats. Involvement of the ß-adrenergic system in regulating SMR2 expression was tested in rats exposed daily to isoproterenol for 4 days. Under these conditions we found an increase in SMR2 expression in female rats, associated with an increase in SMR2 mRNA levels in most acinar cells. This suggests that a signaling cascade, elicited by ß-adrenergic stimuli, might act in concert with androgens to regulate SMR2 expression.

(J Histochem Cytochem 51:1317–1329, 2003)

Key Words: androgens, ß-adrenergic, salivary glands, glutamic acid/glutamine-rich, proteins, transcription regulation, in situ hybridization


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