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Journal of Histochemistry and Cytochemistry, Vol. 51, 1367-1373, October 2003, Copyright © 2003, The Histochemical Society, Inc.

ARTICLE

Localization and Identification of Schistosoma mansoni/KLH-crossreactive Components in Infected Mice

Cecilia Thorsa and Ewert Lindera
a Swedish Institute for Infectious Disease Control, Solna, Sweden, and Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden

Correspondence to: Cecilia Thors, Swedish Institute for Infectious Disease Control (SMI), Solna S-171 82, Sweden. E-mail: cecilia.thors@smi.ki.se

KLH (Keyhole limpet hemocyanin) is highly immunogenic, and crossreactive epitopes occur widely in nature. In schistosomiasis, infected hosts generate antibodies reactive with KLH. This is of diagnostic importance but we lack detailed information on the immunogen-carrying molecules and their distribution in the worm. We used anti-KLH antibodies to localize cross-reacting epitopes in the various developmental stages of the parasite in experimental Schistosoma mansoni infection. The staining results show KLH crossreactivity in the life stages of the parasite. By immunoblotting we show that KLH-crossreactive antigenic epitopes in the parasite eggs are carbohydrates, also recognized by antibodies against soluble schistosome egg antigens. The localizations in the larval stages and in adult worms suggest that crossreacting antigenic epitopes are secretory products.

(J Histochem Cytochem 51:1367–1373, 2003)

Key Words: Schistosoma mansoni, schistosomiasis, keyhole limpet hemocyanin, (KLH), crossreactivity, immunohistochemistry, carbohydrate epitopes, differentiation


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