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Journal of Histochemistry and Cytochemistry
Volume 51 (11): 1467-1478, 2003
Copyright ©The Histochemical Society, Inc.

Morphofunctional Studies of the Glomerular Wall in Mice Lacking Entactin-1

Simon-Philippe Lebel, Yong Chen, Diane Gingras, Albert E. Chung and Moise Bendayan

Department of Pathology and Cell Biology, Université de Montréal, Montreal, Quebec, Canada (S-PL,DG,MB), and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania (YC,AEC)

Correspondence to: M. Bendayan, Dept. of Pathology and Cell Biology, Université de Montréal, CP6128 Succ. Centre Ville, Montreal, Quebec, Canada H3C 3J7. E-mail: moise.bendayan{at}umontreal.ca

The architecture of the basement membranes is essential for proper function. This architecture is based on interactions among its components, which assemble in a complex network. Entactin-1 appears to be the mastermind of this assembling. In entactin-1-null transgenic mice, immunocytochemistry established the absence of entactin-1 in the glomerular basement membrane, and morphological thickening of this membrane was demonstrated. This prompted us to investigate the organization of other components of the glomerular basement membrane in the transgenic animals. The distribution of type IV collagen and laminin remained unchanged, whereas that of anionic charges was significantly altered. We also evaluated the impact of the absence of entactin-1 on cell relays by studying the {alpha}3- and the {alpha}v-integrins along the endothelial and epithelial glomerular cell plasma membranes. Only the density of {alpha}v was found to be increased. Finally, the filtration properties of the glomerular wall were evaluated by revealing endogenous albumin distribution across the basement membrane. This was altered in transgenic animals, suggesting changes in permselectivity properties. Entactin-1 appears to be an essential component in basement membranes because its absence appears to modify the molecular organization leading to alterations in functional properties. (J Histochem Cytochem 51:1467–1478, 2003)

Key Words: entactin-1 • immunocytochemistry • glomerular basement • membrane • collagen type IV • laminin • anionic charges • permselectivity


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