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Journal of Histochemistry and Cytochemistry
Volume 51 (11): 1501-1513, 2003
Copyright ©The Histochemical Society, Inc.

Localization of Cholecystokinin Receptor Subtypes in the Endocine Pancreas

Jean Morisset, Sophie Julien and Jean Lainé

Gastroenterelogy Service, Department of Medicine, Faculty of Medicine, Université de Sherbrooke, Sherbrooke, PQ, Canada

Correspondence to: Dr. Jean Morisset, Gastroenterology Service, Dept. of Medicine, Faculty of Medicine, Université de Sherbrooke, Sherbrooke, PQ, Canada J1H 5N4. E-mail: Jean.Morisset{at}USherbrooke.ca

This study was undertaken to clarify the controversy in the literature about pancreatic localization of the cholecystokinin (CCK) CCKA and CCKB receptors. With antibodies used by other investigators, we first established their specificity by Western blotting, indirect immunofluorescence, and confocal microscopy with each antibody's peptide antigen. Co-localization assays between the CCK receptors and the pancreatic hormones insulin, glucagon, and somatostatin revealed that the CCKA RAbs 1122 and R1–2 recognized insulin and glucagon cells in rat, pig, and human pancreas but not in the somatostatin cells. Conversely, the three CCKB RAbs tested, 9262, 9491, and GR4, identified the somatostatin cells. Abs 9491 and GR4 occasionally co-localized with glucagon, a feature that never occurred with Ab 9262. Finally, the specificity of Ab 9262 for the pancreatic CCKB R was confirmed in six different species. It co-localized with somatostatin but never with glucagon in these species. Our data suggest the use of Abs 1122 and 9262 to specifically identify and localize pancreatic CCKA and CCKB receptors, respectively. Confusion in the literature may result from the lack of specificity of most antibodies used, as established in this study.

(J Histochem Cytochem 51:1501–1513, 2003)

Key Words: pancreas • CCKA–CCKB • receptor localization • islets of Langerhans


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