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Journal of Histochemistry and Cytochemistry
Volume 51 (12): 1689-1698, 2003
Copyright ©The Histochemical Society, Inc.

Expression of Gastric Gland Mucous Cell-Type Mucin in Normal and Neoplastic Human Tissues

Kosei Nakajima, Hiroyoshi Ota, Mu Xia Zhang, Kenji Sano, Takayuki Honda, Keiko Ishii and Jun Nakayama

Institute of Organ Transplants, Reconstructive Medicine and Tissue Engineering, Shinshu University Graduate School of Medicine (KN); Department of Biomedical Laboratory Sciences, School of Health Sciences, School of Medicine, Shinshu University (HO); Department of Laboratory Medicine, School of Medicine, Shinshu University (HO, MXZ,KS,TH,KI,JN); and Department of Pathology, School of Medicine, Shinshu University (JN), Matsumoto, Japan

Correspondence to: Hiroyoshi Ota, Dept. of Biomedical Laboratory Sciences, School of Health Sciences, School of Medicine, Shinshu University, Matsumoto, Nagano 390-8621, Japan. E-mail: hohta{at}gipac.shinshu-u.ac.jp

Gastric gland mucous cells produce class III mucin, which is also found in Brunner's glands and mucous glands along the pancreaticobiliary tract, and in metaplasia and adenocarcinomas differentiating towards gastric mucosa. Recently, we showed that class III mucin possesses GlcNAc{alpha}1->4Galß->R, formed by {alpha}1,4-N-acetylglucosaminyltransferase ({alpha}4GnT). Examining the tissue-specific expression of mucin epitopes is useful to clarify cell-lineage differentiation and to identify the site of origin of metastatic carcinomas in histological specimens. Formalin-fixed, paraffin-embedded tissue sections from esophagus, stomach, colon, liver, pancreas, lung, kidney, prostate, breast, and salivary gland resected for carcinoma, as well as salivary gland adenoma, colon adenoma, and metastatic adenocarcinoma of lymph nodes from stomach, pancreas, colon, and breast, were immunostained for MUC6, {alpha}4GnT, and GlcNAc{alpha}1->4Galß->R. These were all expressed in normal, metaplastic, and adenocarcinoma tissues of stomach, pancreas, and bile duct, and in pulmonary mucinous bronchioloalveolar carcinomas. Cells expressing {alpha}4GnT uniformly expressed GlcNAc{alpha}1->4Galß->R. Only MUC6 was expressed in normal salivary glands, pancreas, seminal vesicles, renal tubules, and colon adenomas, and in normal tissue and adenocarcinomas of prostate and breast. No tissues showed immunoreactivity for {alpha}4GnT alone. Immunohistochemistry (IHC) profiles were similar for metastatic carcinomas and primary carcinoma tissues. The IHC profiles for MUC6, {alpha}4GnT, and GlcNAc{alpha}1->4Galß->R may be diagnostically relevant. (J Histochem Cytochem 51:1689–1698, 2003)

Key Words: glycosyltransferase • gastric mucin • immunohistochemistry • mucin core protein


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