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Journal of Histochemistry and Cytochemistry, Vol. 51, 363-371, March 2003, Copyright © 2003, The Histochemical Society, Inc.

ARTICLE

Expression of the Cystathionine ß Synthase (CBS) Gene During Mouse Development and Immunolocalization in Adult Brain

Karine Roberta, François Vialardb, Eric Thierya, Kiyoko Toyamac, Pierre-Marie Sinetc, Nathalie Janela, and Jacqueline Londona
a EA3508 Université Paris 7, Denis Diderot, Paris, France
b Laboratoire de Cytogénétique, CHU Poissy Saint Germain, Poissy, France
c INSERM U549, IFR Broca Sainte Anne, Centre Paul Broca, Paris, France

Correspondence to: Nathalie Janel, EA3508 Université Paris 7, Denis Diderot, Case 7104, 2 place Jussieu, 75251 Paris Cedex 05, France. E-mail: janel@paris7.jussieu.fr

Hyperhomocysteinemia, caused by a lack of cystathionine ß synthase (CBS), leads to elevated plasma concentrations of homocysteine. This is a common risk factor for atherosclerosis, stroke, and possibly neurodegenerative diseases. However, the mechanisms that link hyperhomocysteinemia due to CBS deficiency to these diseases are still unknown. Early biochemical studies describe developmental and adult patterns of transsulfuration and CBS expression in a variety of species. However, there is incomplete knowledge about the regional and cellular expression pattern of CBS, notably in the brain. To complete the previous data, we used in situ hybridization and Northern blotting to characterize the spatial and temporal patterns of Cbs gene expression during mouse development. In the early stages of development, the Cbs gene was expressed only in the liver and in the skeletal, cardiac, and nervous systems. The expression declined in the nervous system in the late embryonic stages, whereas it increased in the brain after birth, peaking during cerebellar development. In the adult brain, expression was strongest in the Purkinje cell layer and in the hippocampus. Immunohistochemical analyses showed that the CBS protein was localized in most areas of the brain but predominantly in the cell bodies and neuronal processes of Purkinje cells and Ammon's horn neurons. (J Histochem Cytochem 51:363–371, 2003)

Key Words: CBS, homocysteine, development, Purkinje cells, hippocampus, skeletal system


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