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Journal of Histochemistry and Cytochemistry, Vol. 51, 567-574, May 2003, Copyright © 2003, The Histochemical Society, Inc.

ARTICLE

Differential Expression of Ribosomal Proteins in Human Normal and Neoplastic Colorectum

Hide Kasaia,b,c, Daita Nadanoe, Eiko Hidakab, Kayoko Higuchid, Masatomo Kawakuboa,b, Taka-Aki Satof, and Jun Nakayamaa
a Departments of Pathology, Shinshu University School of Medicine, Matsumoto, Japan
b Laboratory Medicine, Shinshu University School of Medicine, Matsumoto, Japan
c Departments of Surgery, Aizawa Hospital, Matsumoto, Japan
d Pathology, Aizawa Hospital, Matsumoto, Japan
e Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan
f Division of Molecular Oncology, Department of Otolaryngology/Head and Neck Surgery and Pathology, College of Physicians and Surgeons, Columbia University, New York, New York

Correspondence to: Jun Nakayama, Dept. of Pathology, Shinshu U. School of Medicine, Asahi 3-1-1, Matsumoto 390-8621, Japan. E-mail: jun@hsp.md.shinshu-u.ac.jp

Ribosomal proteins are a major component of ribosomes and play critical roles in protein biosynthesis. Recently it has been shown that the ribosomal proteins also function during various cellular processes that are independent of protein biosynthesis therefore called extraribosomal functions. In this study we have, for the first time, determined the expression profile of 12 ribosomal proteins (Sa, S8, S11, S12, S18, S24, L7, L13a, L18, L28, L32, and L35a) in normal epithelia of human colorectal mucosa using immunohistochemistry (IHC) and then compared their expression patterns with those of colorectal cancer. In the normal mucosa, ribosomal proteins were largely associated with the ribosomes of mucosal epithelia, and the expression level of ribosomal proteins, except for S11 and L7 proteins, was markedly increased in associated with maturation of the mucosal cells. On the other hand, these ribosomal proteins were markedly decreased in colorectal cancer compared with the normal mucosa. By contrast, S11 and L7 ribosomal proteins were rarely associated with the ribosomes of colorectal epithlia except immature mucosal cells, whereas their expression levels were significantly enchanced in colorectal cancer cells. In addition, L7 ribosomal protien was detected in the secretory granules of the enterochromaffin cells in the colorectal mucosa and in carcinoma cells expressing chromogranin A. These results indicate that the expression of ribosomal proteins is differentially regulated not only in normal mucosa but also in carcinoma of human colorectum, and suggest an extraribosomal function of L7 ribosomal protein in neuroendocrine function.

(J Histochem Cytochem 51:567–573, 2003)

Key Words: ribosome, colorectal cancer, immunohistochemistry


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