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Journal of Histochemistry and Cytochemistry, Vol. 51, 605-612, May 2003, Copyright © 2003, The Histochemical Society, Inc.

ARTICLE

Same Serial Section Correlative Light and Energy-filtered Transmission Electron Microscopy

Ying Rena, Michael J. Kruhlaka, and David P. Bazett–Jonesa
a Programme in Cell Biology, The Hospital for Sick Children, Toronto, Ontario, Canada

Correspondence to: David P. Bazett–Jones, Programme in Cell Biology, The Hospital for Sick Children, 555 University Avenue, Toronto, ONT M5G 1X8, Canada. E-mail: dbjones@sickkids.ca

Correlative imaging of a specific cell with both the light microscope and the electron microscope has proved to be a difficult task, requiring enormous amounts of patience and technical skill. We describe a technique with a high rate of success, which can be used to identify a particular cell in the light microscope and then to embed and thin-section it for electron microscopy. The technique also includes a method to obtain many uninterrupted, thin serial sections for imaging by conventional or energy-filtered transmission electron microscopy, to obtain images for 3D analysis of detail at the suborganelle level.

(J Histochem Cytochem 51:605–612, 2003)

Key Words: fluorescence microscopy, energy-filtered transmission, electron microscopy, electron spectroscopic imaging, nuclear structure, nuclear bodies, promyelocytic leukemia


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