Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Marcos, N. T.
Right arrow Articles by Reis, C. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marcos, N. T.
Right arrow Articles by Reis, C. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 51, 761-771, June 2003, Copyright © 2003, The Histochemical Society, Inc.

ARTICLE

Polypeptide GalNAc-transferases, ST6GalNAc-transferase I, and ST3Gal-transferase I Expression in Gastric Carcinoma Cell Lines

Nuno T. Marcosa, Andrea Cruza, Filipe Silvaa, Raquel Almeidaa, Leonor Davida,b, Ulla Mandelc, Henrik Clausenc, Silvia von Mensdorff–Pouillyd, and Celso A. Reisa
a Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal
b Medical Faculty of the University of Porto, (IPATIMUP) Porto, Portugal
c Department of Oral Diagnostics, School of Dentistry, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark
d Department of Obstetrics and Gynaecology, Academic Hospital Vrije Universiteit, Amsterdam, The Netherlands

Correspondence to: Celso A. Reis, Inst. of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Rua Dr Roberto Frias s/n, 4200 Porto, Portugal. E-mail: celso.reis@ipatimup.pt

Mucin O-glycosylation in cancer is characterized by aberrant expression of immature carbohydrate structures leading to exposure of simple mucin-type carbohydrate antigens and peptide epitopes. Glycosyltransferases controlling the initial steps of mucin O-glycosylation are responsible for the altered glycosylation observed in cancer. We studied the expression in gastric cell lines of six UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GalNAc-T1, T2, T3, T4, T6, T11) that catalyze the initial key step in the regulation of mucin O-glycosylation, the transfer of GalNAc from UDP-GalNAc to serine and threonine residues. We also studied the expression of ST6GalNAc-I, the enzyme responsible for the synthesis of Sialyl-Tn antigen (NeuAc{alpha}2,6GalNAc) and the ST3Gal-I, the enzyme responsible for the synthesis of Sialyl-T antigen (NeuAc{alpha}2,3Galß1,3GalNAc). This study was done using specific monoclonal antibodies, enzymatic assays, and RT-PCR. Our results showed that GalNAc-T1, -T2, and -T3 have an ubiquitous expression in all gastric cell lines, whereas GalNAc-T4, -T6, and -T11 show a restricted expression pattern. The immunoreactivity with MAb VU-2-G7 suggests that, apart from GalNAc-T4, another GalNAc transferase is involved in the glycosylation of the Thr in the PDTR region of the MUC1 tandem repeat. The expression of ST3Gal-I correlates with the expression of the Sialyl-T antigen in gastric cell lines and in the control cell lines studied. The expression of ST6GalNAc-I is low in gastric cell lines, in accordance with the low/absent expression of the Sialyl-Tn antigen.

(J Histochem Cytochem 51:761–771, 2003)

Key Words: GalNAc-transferase, Sialyl-transferase, MUC1, gastric cell lines, mucin O-glycosylation, Sialyl-Tn, Sialyl-T, gastric carcinoma


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 GlycobiologyHome page
T. Freire, R. Lo-Man, F. Piller, V. Piller, C. Leclerc, and S. Bay
Enzymatic large-scale synthesis of MUC6-Tn glycoconjugates for antitumor vaccination
Glycobiology, May 1, 2006; 16(5): 390 - 401.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
N. Berois, D. Mazal, L. Ubillos, F. Trajtenberg, A. Nicolas, X. Sastre-Garau, H. Magdelenat, and E. Osinaga
UDP-N-Acetyl-D-Galactosamine: Polypeptide N-Acetylgalactosaminyltransferase-6 as a New Immunohistochemical Breast Cancer Marker
J. Histochem. Cytochem., March 1, 2006; 54(3): 317 - 328.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
F. Santos-Silva, A. Fonseca, T. Caffrey, F. Carvalho, P. Mesquita, C. Reis, R. Almeida, L. David, and M. A. Hollingsworth
Thomsen-Friedenreich antigen expression in gastric carcinomas is associated with MUC1 mucin VNTR polymorphism
Glycobiology, May 1, 2005; 15(5): 511 - 517.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
N. T. Marcos, S. Pinho, C. Grandela, A. Cruz, B. Samyn-Petit, A. Harduin-Lepers, R. Almeida, F. Silva, V. Morais, J. Costa, et al.
Role of the Human ST6GalNAc-I and ST6GalNAc-II in the Synthesis of the Cancer-Associated Sialyl-Tn Antigen
Cancer Res., October 1, 2004; 64(19): 7050 - 7057.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2003