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Journal of Histochemistry and Cytochemistry, Vol. 51, 1049-1055, August 2003, Copyright © 2003, The Histochemical Society, Inc.

ARTICLE

Membrane-bound Progesterone Receptor Expression in Human Aortic Endothelial Cells

Brenda H. Weltera, Elizabeth L. Hansena, Karla J. Sanera, Yangzhan Weib, and Thomas M. Pricec
a Department of Microbiology and Molecular Medicine, Clemson University, Clemson, South Carolina
b Oncology Research Institute, Greenville Hospital System, Greenville Hospital System, Greenville, South Carolina
c Division of Reproductive Endocrinology, Greenville Hospital System, Greenville, South Carolina

Correspondence to: Thomas M. Price, Duke University Medical Center, Div. of Reproductive Endocrinology and Infertility, Box 3143, Durham, NC 27710. E-mail: price067@notes.duke.edu

Observational studies demonstrate that estradiol and progesterone affect vasoreactivity. In animal studies, progesterone treatment causes immediate relaxation of precontracted arteries with inhibition of calcium influx in vascular endothelial and smooth muscle cells, suggesting a non-genomic mechanism of action. In this study we investigated the presence of novel membrane-bound progesterone receptors in human aortic endothelial cells and correlated the expression with cell-cycle stage. Western blotting analysis with an antibody directed to the hormone-binding domain of the classic progesterone receptors shows predominant bands at 100 and 60 kD, whereas analysis with an antibody to the DNA-binding region shows only the 100-kD band. In contrast, classic nuclear progesterone receptors B and A are identified at 116 and 94 kD in similarly processed T47D cells. Both novel bands localize to the membrane fraction after differential centrifugation. Plasma membrane-bound progesterone receptor was further shown with immunofluorescent antibody and ligand-binding studies in a small percentage of human aortic endothelial cells. Fluorescent activated cell sorting demonstrated that approximately 8% of the human aortic endothelial cells expressed a plasma membrane progesterone receptor and that a greater percentage of the expressing cells were in the G2/M-phase of the cell cycle. Treatment with progesterone conjugated to BSA did not show any significant cell-cycle changes. Plasma membrane-bound progesterone receptor in vascular endothelial cells may regulate the non-genomic actions of progesterone, and expression of the receptor appears to vary with cell cycle stage. (J Histochem Cytochem 51:1049–1055, 2003)

Key Words: progesterone receptor, human aortic endothelial cells, membrane-bound, cell cycle


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