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Journal of Histochemistry and Cytochemistry, Vol. 51, 981-987, August 2003, Copyright © 2003, The Histochemical Society, Inc.

RAPID COMMUNICATION

Combined Tyramide Signal Amplification and Quantum Dots for Sensitive and Photostable Immunofluorescence Detection

Jayne M. Nessa, Rizwan S. Akhtarb,c, Cecelia B. Lathamc, and Kevin A. Rothc
a Division of Pediatric Neurology, Department of Pediatrics, University of Alabama at Birmingham, Birmingham, Alabama
b Department of Neurobiology, University of Alabama at Birmingham, Birmingham, Alabama
c Division of Neuropathology, Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama

Correspondence to: Kevin A. Roth, Div. of Neuropathology, U. of Alabama at Birmingham (UAB), Sparks Center 961E, 1530 Third Avenue South, Birmingham, AL 35294-0017. E-mail: kroth@path.uab.edu

Conventional immunofluorescence detection of biologically relevant proteins and antigens in tissue sections is often limited by relatively weak signals that fade rapidly on illumination. We have developed an immunohistochemical protocol that combines the sensitivity of tyramide signal amplification with the photostability of quantum dots to overcome these limitations. This simple method provides a sensitive and stable fluorescence immunohistochemical alternative to standard chromogen detection.

(J Histochem Cytochem 51:981–987, 2003)

Key Words: immunohistochemistry, photobleaching, tyramide signal amplification, nanocrystals


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