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Journal of Histochemistry and Cytochemistry
Volume 52 (1): 123-130, 2004
Copyright ©The Histochemical Society, Inc.

Distribution of Substance P Receptor (Neurokinin-1 Receptor) in Normal Ovine Lung and During the Progression of Bronchopneumonia in Sheep

Branka Grubor, Rafael Ramirez–Romero, Jack M. Gallup, Ted B. Bailey and Mark R. Ackermann

Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, Iowa (BG,JMG,MRA); Facultad de Medicina Veterinaria y Zootecnia, Universidad Autonoma de Nuevo Leon, Monterrey, Nuevo Leon, Mexico (RR–R); and College of Liberal Arts and Sciences, Iowa State University, Ames, Iowa (TBB)

Correspondence to: Dr. Branka Grubor, Dept. of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1250. E-mail: brankag{at}iastate.edu

Substance P contributes to the physiological homeostasis of pulmonary airways and vasculature. During pneumonia, alterations in substance P production and receptor expression can influence bronchoconstriction and vascular perfusion. The distribution of substance P receptor [neurokinin-1 receptor (NK-1R)] in lungs of normal sheep and sheep with acute (1 day), subacute (15 days), and chronic (45 days) bronchopneumonia caused by Mannheimia haemolytica was determined by immunohistochemistry (IHC). Three rabbit polyclonal antibodies generated to the same cytosolic C-terminal portion of NK-1R (residues 393–407) were tested. NK-1R immunoreactivity was traced in digital images and quantified with IPLAB software. There were no significant differences in NK-1R protein density between normal and infected lambs. Antibody 1 had the broadest distribution and intensity, and stained alveolar septae, smooth muscle cells of airways and vessels, epithelial cells of airways and alveoli, and submucosal glands. When all animals from the study were included, there was a trend towards decreased NK-1R immunoreactivity over time. The work suggests that (a) the density of NK-1R does not change during progression of bacterial (M. haemolytica) bronchopneumonia, (b) NK-1R is widely distributed in ovine lung and decreases with age, and (c) antibodies to the same NK-1R cytosolic region can vary in specificity and affinity. (J Histochem Cytochem 52:123–130, 2004)

Key Words: substance P • NK-1 receptor • ovine lung • immunohistochemistry


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 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E. D. Zee, S. Schomberg, and T. C. Carpenter
Hypoxia upregulates lung microvascular neurokinin-1 receptor expression
Am J Physiol Lung Cell Mol Physiol, July 1, 2006; 291(1): L102 - L110.
[Abstract] [Full Text] [PDF]



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