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Application of Quantum Dots as Probes for Correlative Fluorescence, Conventional, and Energy-filtered Transmission Electron Microscopy

Rozalia Nisman, Graham Dellaire, Ying Ren, Ren Li and David P. Bazett–Jones

Programme in Cell Biology, The Hospital for Sick Children, Toronto, Ontario, Canada

Correspondence to: David P. Bazett–Jones, 555 University Avenue, Toronto, ON M5G 1X8, Canada. E-mail: dbjones{at}sickkids.ca

Luminescent semiconductor quantum dots (QDs) are a new class of fluorescent label with wide-ranging applications for cell imaging. The electron density and elemental composition of these materials permit the extension of their use as probes in conventional electron microscopy (TEM) and energy-filtered TEM (EFTEM). Here we illustrate the feasibility of using streptavidin-conjugated QDs as TEM tags by labeling a nuclear protein on cell sections and obtaining correlative fluorescence and TEM data. We also show that QD probes can be employed in conjunction with immunogold for co-localization of proteins at the ultrastructural level. Furthermore, by obtaining cadmium elemental maps of CdSe/ZnS QDs distributed on a nuclear structure, we demonstrate the potential of QDs for co-localization of multiple proteins when used in combination with EFTEM.

(J Histochem Cystochem 52:13–18, 2004)

Key Words: quantum dots • energy-filtered transmission • electron microscopy • electron spectroscopic imaging • multiple labeling • protein co-localization • cell structure • immunogold

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