Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
DOI: 10.1369/jhc.3A6225.2004
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hilvo, M.
Right arrow Articles by Parkkila, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hilvo, M.
Right arrow Articles by Parkkila, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry
Volume 52 (10): 1313-1322, 2004
Copyright ©The Histochemical Society, Inc.

Expression of Carbonic Anhydrase IX in Mouse Tissues

Mika Hilvo, Monika Rafajová, Silvia Pastoreková, Jaromir Pastorek and Seppo Parkkila

Institute of Medical Technology, University of Tampere and Tampere University Hospital, Tampere, Finland (MH,SP); Centre of Molecular Medicine, Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic (MR,SP,JP); and Department of Clinical Chemistry, University of Oulu, Oulu, Finland (SP)

Correspondence to: Prof. Seppo Parkkila, MD, PhD, Inst. of Medical Technology, University of Tampere, Biokatu 6, 33520 Tampere, Finland. E-mail: Seppo.Parkkila{at}uta.fi

Carbonic anhydrase IX (CA IX) is a unique member of the CA gene family. In contrast to the other isozymes, it has been implicated in regulation of cell proliferation, adhesion, and malignant cell invasion. In a recently described knockout mouse model for CA IX deficiency, the only phenotypic abnormalities were limited to the gastric mucosa, while no changes were observed in the other tissues known to express CA IX in rats and humans. Here we investigated the expression of CA IX mRNA and protein in mouse tissues. Immunohistochemical (IHC) analysis showed strong staining in the gastric mucosa. Moderate reactions were seen in the colon enterocytes and pancreatic acini. The expression pattern of CA IX was similar in certain human and rodent tissues, although some differences existed, especially in the gut epithelium. Reverse transcriptase PCR analyses surprisingly revealed strong signals for CA IX mRNA in the kidney and skeletal muscle, while the IHC and Western blotting showed no or weak signals for the corresponding protein. This result suggests a tight tissue-specific post-transcriptional control for CA IX expression, possibly related to the physiological demands. (J Histochem Cytochem 52:1313–1321, 2004)

Key Words: carbonic anhydrase • expression • mouse • immunohistochemistry • PCR


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. Hilvo, L. Baranauskiene, A. M. Salzano, A. Scaloni, D. Matulis, A. Innocenti, A. Scozzafava, S. M. Monti, A. Di Fiore, G. De Simone, et al.
Biochemical Characterization of CA IX, One of the Most Active Carbonic Anhydrase Isozymes
J. Biol. Chem., October 10, 2008; 283(41): 27799 - 27809.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. J. Scheibe, K. Mundhenk, T. Becker, J. Hallerdei, A. Waheed, G. N. Shah, W. S. Sly, G. Gros, and P. Wetzel
Carbonic anhydrases IV and IX: subcellular localization and functional role in mouse skeletal muscle
Am J Physiol Cell Physiol, February 1, 2008; 294(2): C402 - C412.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
R. J. Scheibe, G. Gros, S. Parkkila, A. Waheed, J. H. Grubb, G. N. Shah, W. S. Sly, and P. Wetzel
Expression of Membrane-bound Carbonic Anhydrases IV, IX, and XIV in the Mouse Heart
J. Histochem. Cytochem., December 1, 2006; 54(12): 1379 - 1391.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. Mizumori, J. Meyerowitz, T. Takeuchi, S. Lim, P. Lee, C. T. Supuran, P. H. Guth, E. Engel, J. D. Kaunitz, and Y. Akiba
Epithelial carbonic anhydrases facilitate PCO2 and pH regulation in rat duodenal mucosa
J. Physiol., June 15, 2006; 573(3): 827 - 842.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
J. A. Haapasalo, K. M. Nordfors, M. Hilvo, I. J. Rantala, Y. Soini, A.-K. Parkkila, S. Pastorekova, J. Pastorek, S. M. Parkkila, and H. K. Haapasalo
Expression of Carbonic Anhydrase IX in Astrocytic Tumors Predicts Poor Prognosis
Clin. Cancer Res., January 15, 2006; 12(2): 473 - 477.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
A. Mayer, M. Hockel, and P. Vaupel
Carbonic Anhydrase IX Expression and Tumor Oxygenation Status Do Not Correlate at the Microregional Level in Locally Advanced Cancers of the Uterine Cervix
Clin. Cancer Res., October 15, 2005; 11(20): 7220 - 7225.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
V. Endeward and G. Gros
Low carbon dioxide permeability of the apical epithelial membrane of guinea-pig colon
J. Physiol., August 15, 2005; 567(1): 253 - 265.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2004