Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

DOI: 10.1369/jhc.4A6252.2004
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sibon, I.
Right arrow Articles by Lamazière, J.-M. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sibon, I.
Right arrow Articles by Lamazière, J.-M. D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry
Volume 52 (11): 1459-1466, 2004
Copyright ©The Histochemical Society, Inc.

Semicarbazide-sensitive Amine Oxidase in Annulo-aortic Ectasia Disease : Relation to Elastic Lamellae-associated Proteins

Igor Sibon, Daniel Larrieu, Khadija el Hadri, Nathalie Mercier, Bruno Fève, Patrick Lacolley, Carlos Labat, Danièle Daret, Jacques Bonnet and Jean-Marie Daniel Lamazière

Inserm U441 (IS,DL,DD,JB,J-MDL), Pessac, France, and CNRS UMR 7079 (KEH,NM,BF) and Inserm EMI U01-07 (CL,PL), Paris, France

Correspondence to: Jean-Marie Daniel Lamazière, Inserm U441, Université Victor Segalen Bordeaux 2, avenue du Haut Lévêque, 33600 Pessac, France. E-mail: jean-marie.d-lamaziere{at}bordeaux.inserm.fr

Lysyl oxidases (Lox), which are members of the amine oxidase family, are involved in the maturation of elastic lamellae and collagen fibers. Modifications of amine oxidases in idiopathic annulo-aortic ectasia disease (IAAED) have never been investigated. Our aim was to examine the expression of several proteins that might interfere with elastic fiber organization in control (n=10) and IAAED (n=18) aortic tissues obtained at surgery. Expression of amine oxidases and semicarbazide-sensitive amine oxidase (SSAO), and cellular phenotypic markers were examined by immunohistopathology and confocal microscopy. The expression of these proteins was assessed in relation to clinical and histomorphological features of the arterial wall. In control aorta, SSAO staining was expressed along elastic lamellae, whereas in aneurysmal areas of IAAED, SSAO was markedly decreased, in association with severe disorganization of elastic lamellae. Smooth muscle myosin heavy chain was also decreased in IAAED compared with controls, indicating smooth muscle cell dedifferentiation. Multiple regression analysis showed that elastic lamellar thickness (ELT) was correlated positively with the SSAO:elastin ratio and negatively with the Lox:elastin ratio, and that the clinical features of IAAED (aneurysm, thoracic aorta diameter, and aortic insufficiency) were positively correlated with ELT but not with SSAO. The relationship between SSAO expression and ELT suggests that this amine oxidase may be involved in elastic fiber organization. However, in advanced IAAED, the deficit in SSAO expression could be secondary to the decrease and fragmentation of elastic fibers and/or to vascular smooth muscle cell dedifferentiation. (J Histochem Cytochem 52:1459–1466, 2004)

Key Words: amine oxidase • aneurysm • idiopathic annulo-aortic ectasia • disease • elastic fiber


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 HypertensionHome page
N. Mercier, K. El Hadri, M. Osborne-Pellegrin, J. Nehme, C. Perret, C. Labat, V. Regnault, J.-M. D. Lamaziere, P. Challande, P. Lacolley, et al.
Modifications of Arterial Phenotype in Response to Amine Oxidase Inhibition by Semicarbazide
Hypertension, July 1, 2007; 50(1): 234 - 241.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
N. Mercier, M. Osborne-Pellegrin, K. El Hadri, A. Kakou, C. Labat, L. Loufrani, D. Henrion, P. Challande, S. Jalkanen, B. Feve, et al.
Carotid arterial stiffness, elastic fibre network and vasoreactivity in semicarbazide-sensitive amine-oxidase null mouse
Cardiovasc Res, November 1, 2006; 72(2): 349 - 357.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2004

 
Purchase HCS Short Course Manual on HCS site