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DOI: 10.1369/jhc.4A6452.2004
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Journal of Histochemistry and Cytochemistry
Volume 52 (12): 1591-1600, 2004
Copyright ©The Histochemical Society, Inc.

A Tracer Study with Systemically and Locally Administered Dinitrophenylated Osteopontin

Antonio Nanci, Rima M. Wazen, Sylvia F. Zalzal, Micheline Fortin, Harvey A. Goldberg, Graeme K. Hunter and Dorin-Lucian Ghitescu

Laboratory for the Study of Calcified Tissues and Biomaterials, Faculty of Dentistry (AN,RMW,SFZ,MF) and Department of Pathology and Cell Biology, Faculty of Medicine (D-LG), Université de Montréal, Montreal, QC, Canada, and CIHR Group in Skeletal Development and Remodeling, School of Dentistry (HAG,GKH), University of Western Ontario, ON, Canada

Correspondence to: Antonio Nanci, Laboratory for the Study of Calcified Tissues and Biomaterials, Faculty of Dentistry, Université de Montréal, PO Box 6128, Station Centre-Ville, Montreal, QC, Canada H3C 3J7. E-mail: antonio.nanci{at}umontreal.ca

Osteopontin (OPN), a major non-collagenous matrix protein of bone, is also found in tissue fluids and in the circulation. It is still not clear whether circulating OPN contributes to bone formation. To elucidate this question, rat OPN was tagged with dinitrophenol groups and administered to rats either intravenously or by infusion with an osmotic minipump through a "surgical window" in the bone of the hemimandible. Dinitrophenylated rat albumin (ALB) was used as a control. The presence and distribution of tagged proteins were revealed by immunogold labeling on sections of tibia and alveolar bone. Tagged molecules of OPN were found in mineralization foci, surfaces and interfaces, and matrix accumulations among calcified collagen fibrils. Even though dinitrophenylated ALB was administered at several-fold higher concentrations, it did not accumulate in these sites. These results show that circulating OPN can be incorporated into specific compartments of forming bone and suggest that such molecules may play a more important role than previously suspected. (J Histochem Cytochem 52:1591–1600, 2004)

Key Words: tracer • immunocytochemistry • osteopontin • albumin


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