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DOI: 10.1369/jhc.4A6330.2004
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Journal of Histochemistry and Cytochemistry
Volume 52 (12): 1609-1617, 2004
Copyright ©The Histochemical Society, Inc.

Pattern of Expression of HtrA1 During Mouse Development

Antonio De Luca1, Maria De Falco1, Luca De Luca, Roberta Penta, Viji Shridhar, Feliciano Baldi, Mara Campioni, Marco G. Paggi and Alfonso Baldi

Department of Medicine and Public Health, Section of Clinical Anatomy, Second University of Naples (ADL,LDL,RP), Naples, Italy; Department of Evolutive and Comparative Biology, University of Naples "Federico II," (MDF), Naples, Italy; Department of Biochemistry and Biophysics "F. Cedrangolo," Section of Pathologic Anatomy, Second University of Naples (FB,AB), Naples, Italy; Department of Experimental Pathology, Mayo Clinic Cancer Center, Rochester, Minnesota (VS); and Laboratory "C," Department for the Development of Therapeutic Programs, Center for Experimental Research, Regina Elena Institute, Rome, Italy (MC,MGP)

Correspondence to: Dr. Alfonso Baldi, Dept. of Biochemistry and Biophysics "F. Cedrangolo," Section of Pathologic Anatomy, Second University of Naples, Via L. Armanni 5, 80138 Naples, Italy. E-mail: alfonsobaldi{at}tiscali.it

The human HtrA family of proteases consists of four members: HtrA1, HtrA2, HtrA3, and HtrA4. In humans the four HtrA homologues appear to be involved in several important functions such as cell growth, apoptosis, and inflammatory reactions, and they control cell fate via regulated protein metabolism. In previous studies it was shown that the expression of HtrA1 was ubiquitous in normal adult human tissues. Here we examined the expression of HtrA1 protein and its corresponding mRNA during mouse embryogenesis using Northern blotting hybridization, RT-PCR, and immunohistochemical staining analyses. Our results indicate that HtrA1 is expressed in a variety of tissues in mouse embryos. Furthermore, this expression is regulated in a spatial and temporal manner. Relatively low levels of HtrA1 mRNA are detected in embryos at the beginning of organogenesis (E8), and the levels of expression increase during late organogenesis (E14–E19). Our results show that HtrA1 was expressed during embryonic development in specific areas where signaling by TGFß family proteins plays important regulatory roles. The expression of HtrA1, documented both at mRNA and protein levels by RT-PCR and immunohistochemistry in the developing nervous system, is consistent with a possible role of this protein both in dividing and postmitotic neurons, possibly via its documented inhibitory effects on TGFß proteins. An exhaustive knowledge of the different cell- and tissue-specific patterns of expression of HtrA1 in normal mouse embryos is essential for a critical evaluation of the exact role played by this protein during development. (J Histochem Cytochem 52:1609–1617, 2004)

Key Words: HtrA1 • expression in mouse embryo • immunohistochemistry • RT-PCR • Northern blotting


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S. Grau, A. Baldi, R. Bussani, X. Tian, R. Stefanescu, M. Przybylski, P. Richards, S. A. Jones, V. Shridhar, T. Clausen, et al.
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