|
|
|
|
 |
|||
|
|||
Volume 52 (2): 169-177, 2004
Copyright ©The Histochemical Society, Inc.
Cocaine- and Amphetamine-regulated Transcript (CART) Is Expressed in Several Islet Cell Types During Rat Development
Departments of Physiological Sciences (NW,BON,EE,FS) and Cell and Molecular Biology (HM), Lund University, Lund, Sweden, and Yerkes National Primate Research Center (MK), Emory University, Atlanta, Georgia
Correspondence to: Nils Wierup, Lund University, Dept. of Physiological Sciences, Sect. of Neuroendocrine Cell Biology, BMC F10, 22 184 Lund, Sweden. E-mail: nils.wierup{at}mphy.lu.se
Cocaine- and amphetamine-regulated transcript (CART) is an anorexigenic peptide widely expressed in the central and peripheral, including the enteric, nervous systems. CART is also expressed in pituitary endocrine cells, adrenomedullary cells, islet somatostatin cells, and in rat antral gastrin cells. We used immunocytochemistry (IHC) and in situ hybridization (ISH) to study CART expression in developing rat pancreas. We also examined co-expression of CART and islet hormones and developmental markers and the effect of CART on proliferation using clonal insulin cells (INS-1 832/13). A major portion of each of the islet cell types, except the ghrelin cells, expressed CART during a period before and around birth. Two weeks postnatally, CART expression was restricted to somatostatin cells. Pre- and early postnatally, many of the CART-expressing cells co-expressed cytokeratin 20 (CK20), a marker of duct cells and islet precursor cells, the trophic hormone gastrin, and a smaller subpopulation also harbored the proliferation marker Ki67. CART was also expressed in pancreatic nerve fibers, both sensory and autonomic, and in ganglion nerve cell bodies. Although highly expressed in the developing islets, CART did not affect proliferation of INS-1 cells. We have demonstrated that CART is expressed in several islet cell types during rat development but is restricted to somatostatin cells and neurons in the adult rat.
(J Histochem Cytochem 52:169–177, 2004)
Key Words: CART • cocaine- amphetamine- regulated transcript • pancreatic nerves • islet development • INS-1 (832/13)-cells • ghrelin • gastrin • VIP • islet hormones • neuropeptides
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Borg, C. Klint, N. Wierup, K. Strom, S. Larsson, F. Sundler, R. Lupi, P. Marchetti, G. Xu, A. Kimmel, et al. Perilipin Is Present in Islets of Langerhans and Protects against Lipotoxicity When Overexpressed in the {beta}-Cell Line INS-1 Endocrinology, July 1, 2009; 150(7): 3049 - 3057. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Sen, L. Lv, N. Bello, J. J. Ireland, and G. W. Smith Cocaine- and Amphetamine-Regulated Transcript Accelerates Termination of Follicle-Stimulating Hormone-Induced Extracellularly Regulated Kinase 1/2 and Akt Activation by Regulating the Expression and Degradation of Specific Mitogen-Activated Protein Kinase Phosphatases in Bovine Granulosa Cells Mol. Endocrinol., December 1, 2008; 22(12): 2655 - 2676. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. V. Ivanova, R. Schubert, D. B. Duridanova, T. B. Bolton, L. T. Lubomirov, and H. S. Gagov Heart/Cardiac Muscle: Cocaine- and amphetamine-regulated transcript (CART) peptide as an in vivo regulator of cardiac function in Rana ridibunda frog Exp Physiol, November 1, 2007; 92(6): 1037 - 1046. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Arumugam, D. Fleenor, and M. Freemark Lactogenic and Somatogenic Hormones Regulate the Expression of Neuropeptide Y and Cocaine- and Amphetamine-Regulated Transcript in Rat Insulinoma (INS-1) Cells: Interactions with Glucose and Glucocorticoids Endocrinology, January 1, 2007; 148(1): 258 - 267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Ahren, N. Wierup, and F. Sundler Neuropeptides and the Regulation of Islet Function Diabetes, December 1, 2006; 55(Supplement_2): S98 - S107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Ahn, B. Dubern, C. Lubrano-Berthelier, K. Clement, and G. Karsenty Cart Overexpression Is the Only Identifiable Cause of High Bone Mass in Melanocortin 4 Receptor Deficiency Endocrinology, July 1, 2006; 147(7): 3196 - 3202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Smith, J. M. Vaughan, C. J. Donaldson, R. E. Fernandez, C. Li, A. Chen, and W. W. Vale Cocaine- and Amphetamine-Regulated Transcript Is Localized in Pituitary Lactotropes and Is Regulated during Lactation Endocrinology, March 1, 2006; 147(3): 1213 - 1223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Wierup, M. Bjorkqvist, M. J. Kuhar, H. Mulder, and F. Sundler CART Regulates Islet Hormone Secretion and Is Expressed in the {beta}-Cells of Type 2 Diabetic Rats Diabetes, February 1, 2006; 55(2): 305 - 311. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Smith, J. M. Vaughan, C. J. Donaldson, J. Rivier, C. Li, A. Chen, and W. W. Vale Cocaine- and Amphetamine-Regulated Transcript Activates the Hypothalamic-Pituitary-Adrenal Axis through a Corticotropin-Releasing Factor Receptor-Dependent Mechanism Endocrinology, November 1, 2004; 145(11): 5202 - 5209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Vicentic, G. Dominguez, R. G. Hunter, K. Philpot, M. Wilson, and M. J. Kuhar Cocaine- and Amphetamine-Regulated Transcript Peptide Levels in Blood Exhibit a Diurnal Rhythm: Regulation by Glucocorticoids Endocrinology, September 1, 2004; 145(9): 4119 - 4124. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |
