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Journal of Histochemistry and Cytochemistry
Volume 52 (2): 179-185, 2004
Copyright ©The Histochemical Society, Inc.

Frequency of M-Cadherin-stained Satellite Cells Declines in Human Muscles During Aging

Spela Sajko, Lucie Kubínová, Erika Cvetko, Marko Kreft, Anton Wernig and Ida Erzen

Institutes of Anatomy (SS,EC,IE) and of Pathophysiology (MK), Medical Faculty, Ljubljana, Slovenia; Department of Biomathematics (LK), Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic; and Department of Physiology (AW), University of Bonn, Bonn, Germany

Correspondence to: Dr. Ida Erzen, Scientific Councilor, Institute of Anatomy, Medical Faculty, Korytkova 2, 1000 Ljubljana, Slovenia. E-mail: ida.erzen{at}mf.uni-lj.si

To answer the question of whether the satellite cell pool in human muscle is reduced during aging, we detected satellite cells in 30-µm-thick transverse sections under the confocal microscope by binding of M-cadherin antibody. The basal lamina was detected with laminin. Nuclei were stained with bisbenzimide or propidium iodide. Satellite cells were counted by applying the disector method and unbiased sampling design. To determine if there are age-related differences in muscle fiber types, morphometric characteristics of muscle fibers were examined on thin sections stained for myofibrillar ATPase. Autopsy samples of vastus lateralis muscle from six young (28.7 ± 2.3 years) and six old (70.8 ± 1.3 years) persons who had suffered sudden death were analyzed. Numbers of satellite cells per fiber length (Nsc/Lfib) and number of satellite cells per total number of nuclei (satellite cell nuclei + myonuclei) (Nsc/Nnucl) were significantly lower in the old group (p< 0.05). We demonstrate the importance of proper sampling and counting in estimation of sparsely distributed structures such as satellite cells. Our results support the hypothesis that the satellite cell fraction declines during aging. (J Histochem Cytochem 52:179–185, 2004)

Key Words: aging • human • satellite cells • skeletal muscle • stereology


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