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Journal of Histochemistry and Cytochemistry
Volume 52 (2): 211-216, 2004
Copyright ©The Histochemical Society, Inc.

Silver Carbonate Staining Reveals Mitochondrial Heterogeneity

José M. López–Cepero

Department of Cell Biology, Histology and Pathology, Faculty of Medicine, University of Cádiz, Spain, and Department of Pathology II, Faculty of Health Sciences, University Hospital, Linköping, Sweden

Correspondence to: Prof. José M. López–Cepero, Departamento de Anatomía Patológica, Biología Celular e Histología, Facultad de Medicina, Universidad de Cádiz, Plaza del Falla 9, 11003 Cádiz, Spain. E-mail: jose.lopezcepero{at}uca.es

Silver staining methods, when selective, yield a high-contrast and high-resolution image in optical microscopy. A classical method for silver impregnation of mitochondria has been applied to murine tissues and reveals a marked heterogeneity among mitochondria in single cells. This heterogeneity can be detected in the optical microscope but is even more evident at the ultrastructural level. The differences in staining intensity may reflect different stages in the mitochondrial life cycle. The progressive accumulation of uranyl–argyrophilic material may be a marker of mitochondrial aging. This highly selective staining procedure may be of use in studies of mitochondrial changes under pathological conditions and during apoptosis. (J Histochem Cytochem 52:211–216, 2004)

Key Words: mitochondria • silver carbonate • mitochondrial life cycle • mitochondrial heterogeneity • mitochondrial fusion


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