|
|
|
|
 |
|||
|
|||
Volume 52 (3): 301-310, 2004
Copyright ©The Histochemical Society, Inc.
Ghrelin Is Expressed in a Novel Endocrine Cell Type in Developing Rat Islets and Inhibits Insulin Secretion from INS-1 (832/13) Cells
Departments of Physiological Sciences (NW,FS) and Cell and Molecular Biology (SY,HM), Lund University, Lund, Sweden, and Department of Medicine (RJM), University of California, San Diego, California
Correspondence to: Nils Wierup, Dept. of Physiological Sciences, Section of Neuroendocrine Cell Biology, BMC F10, Lund University, 22 184 Lund, Sweden. E-mail: nils.wierup{at}mphy.lu.se
Ghrelin is produced mainly by endocrine cells in the stomach and is an endogenous ligand for the growth hormone secretagogue receptor (GHS-R). It also influences feeding behavior, metabolic regulation, and energy balance. It affects islet hormone secretion, and expression of ghrelin and GHS-R in the pancreas has been reported. In human islets, ghrelin expression is highest pre- and neonatally. We examined ghrelin and GHS-R in rat islets during development with immunocytochemistry and in situ hybridization. We also studied the effect of ghrelin on insulin secretion from INS-1 (832/13) cells and the expression of GHS-R in these cells. We found ghrelin expression in rat islet endocrine cells from mid-gestation to 1 month postnatally. Islet expression of GHS-R mRNA was detected from late fetal stages to adult. The onset of islet ghrelin expression preceded that of gastric ghrelin. Islet ghrelin cells constitute a separate and novel islet cell population throughout development. However, during a short perinatal period a minor subpopulation of the ghrelin cells co-expressed glucagon or pancreatic polypeptide. Markers for cell lineage, proliferation, and duct cells revealed that the ghrelin cells proliferate, originate from duct cells, and share lineage with glucagon cells. Ghrelin dose-dependently inhibited glucose-stimulated insulin secretion from INS-1 (832/13) cells, and GHS-R was detected in the cells. We conclude that ghrelin is expressed in a novel developmentally regulated endocrine islet cell type in the rat pancreas and that ghrelin inhibits glucose-stimulated insulin secretion via a direct effect on the ß-cell. (J Histochem Cytochem 52:301–310, 2004)
Key Words: ghrelin • islet hormones • islet cells • islet development • insulin • ß-cells • INS-1 (823/13) cells • Brn 4
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. A. Gutierrez, P. J. Solenberg, D. R. Perkins, J. A. Willency, M. D. Knierman, Z. Jin, D. R. Witcher, S. Luo, J. E. Onyia, and J. E. Hale From the Cover: Ghrelin octanoylation mediated by an orphan lipid transferase PNAS, April 29, 2008; 105(17): 6320 - 6325. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. P. Esler, J. Rudolph, T. H. Claus, W. Tang, N. Barucci, S.-E. Brown, W. Bullock, M. Daly, L. DeCarr, Y. Li, et al. Small-Molecule Ghrelin Receptor Antagonists Improve Glucose Tolerance, Suppress Appetite, and Promote Weight Loss Endocrinology, November 1, 2007; 148(11): 5175 - 5185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Wierup, M. Bjorkqvist, B. Westrom, S. Pierzynowski, F. Sundler, and K. Sjolund Ghrelin and Motilin Are Cosecreted from a Prominent Endocrine Cell Population in the Small Intestine J. Clin. Endocrinol. Metab., September 1, 2007; 92(9): 3573 - 3581. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Dezaki, M. Kakei, and T. Yada Ghrelin Uses G{alpha}i2 and Activates Voltage-Dependent K+ Channels to Attenuate Glucose-Induced Ca2+ Signaling and Insulin Release in Islet {beta}-Cells: Novel Signal Transduction of Ghrelin Diabetes, September 1, 2007; 56(9): 2319 - 2327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Gauna, R. M. Kiewiet, J. A. M. J. L. Janssen, B. van de Zande, P. J. D. Delhanty, E. Ghigo, L. J. Hofland, A. P. N. Themmen, and A. J. van der Lely Unacylated ghrelin acts as a potent insulin secretagogue in glucose-stimulated conditions Am J Physiol Endocrinol Metab, September 1, 2007; 293(3): E697 - E704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Wang, L. Liang, and L. Du The effects of intrauterine undernutrition on pancreas ghrelin and insulin expression in neonate rats J. Endocrinol., July 1, 2007; 194(1): 121 - 129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Fak, L. Friis-Hansen, B. Westrom, and N. Wierup Gastric ghrelin cell development is hampered and plasma ghrelin is reduced by delayed weaning in rats J. Endocrinol., February 1, 2007; 192(2): 345 - 352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Gromada, I. Franklin, and C. B. Wollheim {alpha}-Cells of the Endocrine Pancreas: 35 Years of Research but the Enigma Remains Endocr. Rev., February 1, 2007; 28(1): 84 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Granata, F. Settanni, L. Biancone, L. Trovato, R. Nano, F. Bertuzzi, S. Destefanis, M. Annunziata, M. Martinetti, F. Catapano, et al. Acylated and Unacylated Ghrelin Promote Proliferation and Inhibit Apoptosis of Pancreatic {beta}-Cells and Human Islets: Involvement of 3',5'-Cyclic Adenosine Monophosphate/Protein Kinase A, Extracellular Signal-Regulated Kinase 1/2, and Phosphatidyl Inositol 3-Kinase/Akt Signaling Endocrinology, February 1, 2007; 148(2): 512 - 529. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhu, Y. Cao, K. Voodg, and D. F. Steiner On the Processing of Proghrelin to Ghrelin J. Biol. Chem., December 15, 2006; 281(50): 38867 - 38870. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Dezaki, H. Sone, M. Koizumi, M. Nakata, M. Kakei, H. Nagai, H. Hosoda, K. Kangawa, and T. Yada Blockade of Pancreatic Islet-Derived Ghrelin Enhances Insulin Secretion to Prevent High-Fat Diet-Induced Glucose Intolerance Diabetes, December 1, 2006; 55(12): 3486 - 3493. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Bertelli and M. Bendayan Association between Endocrine Pancreas and Ductal System. More than an Epiphenomenon of Endocrine Differentiation and Development? J. Histochem. Cytochem., September 1, 2005; 53(9): 1071 - 1086. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. D Bruder, L. Jacobson, and H. Raff Plasma leptin and ghrelin in the neonatal rat: interaction of dexamethasone and hypoxia J. Endocrinol., June 1, 2005; 185(3): 477 - 484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Iwakura, K. Hosoda, C. Son, J. Fujikura, T. Tomita, M. Noguchi, H. Ariyasu, K. Takaya, H. Masuzaki, Y. Ogawa, et al. Analysis of Rat Insulin II Promoter-Ghrelin Transgenic Mice and Rat Glucagon Promoter-Ghrelin Transgenic Mice J. Biol. Chem., April 15, 2005; 280(15): 15247 - 15256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kojima and K. Kangawa Ghrelin: Structure and Function Physiol Rev, April 1, 2005; 85(2): 495 - 522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Cruciani-Guglielmacci, M. Vincent-Lamon, C. Rouch, M. Orosco, A. Ktorza, and C. Magnan Early changes in insulin secretion and action induced by high-fat diet are related to a decreased sympathetic tone Am J Physiol Endocrinol Metab, January 1, 2005; 288(1): E148 - E154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Dezaki, H. Hosoda, M. Kakei, S. Hashiguchi, M. Watanabe, K. Kangawa, and T. Yada Endogenous Ghrelin in Pancreatic Islets Restricts Insulin Release by Attenuating Ca2+ Signaling in {beta}-Cells: Implication in the Glycemic Control in Rodents Diabetes, December 1, 2004; 53(12): 3142 - 3151. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Rindi, A. Torsello, V. Locatelli, and E. Solcia Ghrelin Expression and Actions: A Novel Peptide for an Old Cell Type of the Diffuse Endocrine System Experimental Biology and Medicine, November 1, 2004; 229(10): 1007 - 1016. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-P. Chanoine and A. C. K. Wong Ghrelin Gene Expression Is Markedly Higher in Fetal Pancreas Compared with Fetal Stomach: Effect of Maternal Fasting Endocrinology, August 1, 2004; 145(8): 3813 - 3820. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |