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Journal of Histochemistry and Cytochemistry
Volume 52 (3): 415-418, 2004
Copyright ©The Histochemical Society, Inc.

BRIEF REPORT

Calcium-activated Chloride Channel-2 in Human Epithelia

Che J. Connon, Kenta Yamasaki, Satoshi Kawasaki, Andrew J. Quantock, Noriko Koizumi and Shigeru Kinoshita

Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kawaramachi, Kamigyo-ku, Kyoto, Japan (CJC,KY,SK,NK,SK), School of Optometry and Vision Sciences (CJC,AJQ), and Cardiff Institute of Tissue Engineering and Repair (CJC,AJQ), Cardiff University, Cardiff, Wales, United Kingdom

Correspondence to: Che J. Connon, Dept. of Ophthalmology, Kyoto Prefectural University of Medicine, Kawaramachi, Kamigyo-ku, Kyoto, Japan, 602-0841. E-mail: connoncj{at}cf.ac.uk

Calcium-activated chloride channels (CLCAs) are a family of multifunctional proteins that are widely distributed in tissues. To investigate the distribution of human CLCA-2 (hCLCA2) in human epithelia at the light and electron microscopic levels, we raised a primary antibody against a synthetic polypeptide sequence from natural hCLCA2. Corneal, skin, vaginal, esophageal, and laryngeal epithelia were immunopositive for hCLCA2 at the cytosolic aspect of the basal cells adjacent to the basement membrane. Epithelia of stomach and small intestine showed no hCLCA2 immunoreactivity. This study reports the cellular distribution of hCLCA2 in human epithelia and suggests its possible involvement in epithelial stratification and cell–substrate adhesion.

(J Histochem Cytochem 52:415–418, 2004)

Key Words: human • hCLCA2 • epithelium • immunohistochemistry • electron microscopy


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