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Journal of Histochemistry and Cytochemistry
Volume 52 (4): 545-548, 2004
Copyright ©The Histochemical Society, Inc.

BRIEF REPORT

Immunohistochemical and mRNA Localization of the Angiotensin II Receptor Subtype 2 (AT2) in Follicular Granulosa Cells of the Rat Ovary

Nicholas Obermüller, Monika Gentili, Stefan Gauer, Norbert Gretz, Michael Weigel, Helmut Geiger and Nikolaus Gassler

Division of Nephrology, IV Medical Clinic (NO,SG,HG), University of Frankfurt, Frankfurt, Germany; Medical Research Center (NGr) and Department of Obstetrics and Gynecology (MG,MW), Klinikum Mannheim, University of Heidelberg, Mannheim; and Institute of Pathology (NGa), University of Heidelberg, Heidelberg, Germany

Correspondence to: Dr. Nicholas Obermüller, Div. of Nephrology/IV Medical Clinic, University of Frankfurt, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany. E-mail: obermueller{at}em.uni-frankfurt.de

A local renin–angiotensin system (RAS), including specific angiotensin II receptor subtypes, is present in the rat ovary. Immunohistochemistry using a polyclonal antibody and mRNA in situ hybridization were performed on perfusion-fixed, paraffin-embedded ovaries obtained from untreated sexually mature, normally cycling rats. Immunofluorescent staining revealed an exclusive and distinct labeling of follicular granulosa cells showing a plaque-like expression pattern at the cell borders, being detectable in different stages of atretic degeneration. On adjacent sections the expression of the respective mRNA could be shown in granulosa cells of the same follicle. The AT2 receptor may be implicated in the ovarian atretic process by influencing follicular cell–cell communication.

(J Histochem Cytochem 52:545–548, 2004)

Key Words: renin–angiotensin system (RAS) • AT2 receptor • immunohistochemistry • granulosa cells • atretic follicle • rat ovary


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