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Immunohistochemical Study of Protein 4.1B in the Normal and W/W^v Mouse Seminiferous Epithelium

Nobuo Terada, Nobuhiko Ohno, Hisashi Yamakawa, Takeshi Baba, Yasuhisa Fujii, Zagreb Zea, Osamu Ohara and Shinichi Ohno

Department of Anatomy, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Tamaho, Japan (NT,NO,TB,YF,ZZ,SO), and Department of Human Gene Research, Kazusa DNA Research Institute, Kisarazu, Japan (HY,OO)

Correspondence to: Nobuo Terada, MD, PhD, Dept. of Anatomy, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Tamaho, Yamanashi 409-3898, Japan. E-mail: nobuot{at}yamanashi.ac.jp

Cell–cell adhesion is crucial not only for mechanical adhesion but also for tissue morphogenesis. Protein 4.1B, a member of the protein 4.1 family named from an erythrocyte membrane protein, is a potential organizer of an adherens system. In adult mouse seminiferous tubules, protein 4.1B localized in the basal compartment, especially in the attaching region of spermatogonia and Sertoli cells. Protein 4.1B localization and appearance were not different in each spermatogenic stage. Developmentally, protein 4.1B was not detected at postnatal day 3 (P3), was diffusely localized at P15, and was found in the basal compartment during the third week. By double staining for protein 4.1B and F-actin, their localizations were shown to be different, indicating that protein 4.1B was localized in a region lower than the basal ectoplasmic specialization that formed the Sertoli–Sertoli junction. By electron microscopy, immunoreactive products were seen mainly on the membranes of Sertoli cells. In the W/W^v mutant mouse, the seminiferous epithelium had few germ cells. Protein 4.1B and ß-catenin were not detected, although the basal ectoplasmic specialization was retained. These results indicate that protein 4.1B may be related to the adhesion between Sertoli cells and germ cells, especially the spermatogonium.

(J Histochem Cytochem 52:769–777, 2004)

Key Words: testis • spermatogonium • Sertoli cell • cell–cell adhesion • junction

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