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DOI: 10.1369/jhc.3A6177.2004
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Journal of Histochemistry and Cytochemistry
Volume 52 (7): 873-883, 2004
Copyright ©The Histochemical Society, Inc.

Early Regeneration of Whole Skeletal Muscle Grafts Is Unaffected by Overexpression of IGF-1 in MLC/mIGF-1 Transgenic Mice

Thea Shavlakadze, Marilyn Davies, Jason D. White and Miranda D. Grounds

School of Anatomy and Human Biology, University of Western Australia, Crawley, Perth, Western Australia (TS,MD,JDW,MDG)

Correspondence to: Miranda Grounds, School of Anatomy and Human Biology, Crawley, Western Australia 6009. E-mail: mgrounds{at}anhb.uwa.edu.au

Early myogenic events in regenerating whole muscle grafts were compared between transgenic MLC/mIGF-1 mice with skeletal muscle-specific overexpression of the Exon-1 Ea isoform of insulin-like growth factor-1 (mIGF-1) and control FVB mice, from day 3 to day 21 after transplantation. Immunocytochemistry with antibodies against desmin showed that skeletal muscle-specific overexpression of IGF-1 did not affect the pattern of myoblast activation or proliferation or the onset and number of myotubes formed in regenerating whole muscle grafts. Hypertrophied myotubes were observed in MLC/mIGF grafts at day 7 after transplantation, although such hypertrophy was transient, and the transgenic and control grafts had a similar appearance at later time points (days 10, 14, and 21). Immunostaining with antibodies to platelet endothelial cell adhesion molecule-1, which identifies endothelial cells, demonstrated no difference in the formation of new vascular network in grafts of transgenic and control mice. Skeletal muscle-specific overexpression of mIGF-1 does not appear to stimulate the early events associated with myogenesis during regeneration of whole muscle grafts. (J Histochem Cytochem 52:873–883, 2004)

Key Words: muscle regeneration • myogenesis • IGF-1 • transgenic mice • transplantation • vascular network


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