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Volume 52 (7): 893-901, 2004
Copyright ©The Histochemical Society, Inc.
Immunohistochemical Detection of EGFR in Paraffin-embedded Tumor Tissues : Variation in Staining Intensity Due to Choice of Fixative and Storage Time of Tissue Sections
University Witten/Herdecke, Institute of Pathology, Wuppertal, Germany (DA,SS); Merck KGaA, Darmstadt, Germany (K-AR); DakoCytomation A/S, Glostrup, Denmark (CLT,HW); and University of Applied Sciences, Münster, Germany (MSB)
Correspondence to: Prof. Dr. Med. S. Störkel, Inst. of Pathology, University Witten/Herdecke, Helios-Klinikum, Wuppertal, Heusnerstr.40, 42283 Wuppertal, Germany. E-mail: sstoerkel{at}wuppertal.helios-kliniken.de
The epidermal growth factor receptor (EGFR) is highly expressed in a variety of solid malignant tumors and its expression has been correlated with disease progression and poor survival. With the advent of targeted therapies, especially IMC-C225 (Cetuximab), a monoclonal antibody (MAb) directed against the EGFR, there is an increasing interest in immunohistochemistry (IHC)-based EGFR screening methods using paraffin-embedded tumor specimens to select cancer patients eligible for treatment with Cetuximab. With the EGFRpharmDX kit, a complete assay for demonstration of EGFR is now available. Because no information about the preservation of the EGFR under various conditions of fixation is available, we performed a prospective study on a panel of commonly used fixatives to determine optimal tissue preservation protocols. The stability of the epitope on cut tissue sections stored for a period up to 24 month was also tested using material originating from patients with head and neck cancer, non-small-cell lung carcinomas, and colorectal adenocarcinomas. Depending on the fixative used and the time of storage of cut tissue sections, a variation in the determined level of EGFR expression was demonstrated compared with the most optimal fixation procedure. (J Histochem Cytochem 52:893–901, 2004)
Key Words: immunohistochemistry • tissue preservation • fixation protocols • EGFR • stored slides
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