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DOI: 10.1369/jhc.4B6271.2004
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Journal of Histochemistry and Cytochemistry
Volume 52 (7): 985-989, 2004
Copyright ©The Histochemical Society, Inc.

BRIEF REPORT

25-Hydroxyvitamin D3-1{alpha}-hydroxylase Expression in Normal and Malignant Human Colon

Giovanna Bises, Enikö Kállay, Tina Weiland, Friedrich Wrba, Etienne Wenzl, Elisabeth Bonner, Stefan Kriwanek, Peter Obrist and Heide S. Cross

Departments of Pathophysiology (GB,EK,TW,HSC), Clinical Pathology (FW), and Surgery (EW), Medical University of Vienna, Vienna, Austria; Departments of Pathology (EB) and Surgery (SK), Hospital Rudolfstiftung, Vienna, Austria; and Institute of Pathology (PO), University of Innsbruck, Innsbruck, Austria

Correspondence to: Dr. Heide S. Cross, Dept. of Pathophysiology, Medical University of Vienna, Waehringerguertel 18-20, A-1090 Vienna, Austria. E-mail: heide.cross{at}akh-wien.ac.at

1,25-dihydroxyvitamin D3 has anti-mitotic, pro-differentiating, and pro-apoptotic activity in tumor cells. We demonstrated that the secosteroid can be synthesized and degraded not only in the kidney but also extrarenally in intestinal cells. Evaluation of 1,25-dihydroxyvitamin D3-synthesizing CYP27B1 hydroxylase mRNA (real-time PCR) and protein (immunoblotting, immunofluorescence) showed enhanced expression in high- to medium-differentiated human colon tumors compared with tumor-adjacent normal mucosa or with colon mucosa from non-cancer patients. In high-grade undifferentiated tumor areas expression was lost. Many cells co-expressed CYP27B1 and the vitamin D receptor. We suggest that autocrine/paracrine antimitotic activity of 1,25-dihydroxyvitamin D3 could prevent intestinal tumor formation and progression. (J Histochem Cytochem 52:985–989, 2004)

Key Words: extrarenal vitamin D synthesis • colorectal cancer • CYP27B1 (25-hydroxyvitamin D3-1{alpha}-hydroxylase) • vitamin D receptor • immunofluorescence • real-time PCR • differentiated colon tumor cells


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