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DOI: 10.1369/jhc.3A6213.2004
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Journal of Histochemistry and Cytochemistry
Volume 52 (8): 1031-1036, 2004
Copyright ©The Histochemical Society, Inc.

Differential Expression of Apolipoprotein D in Human Astroglial and Oligodendroglial Cells

Ana Navarro, Eva del Valle and Jorge Tolivia

Department of Morphology and Cell Biology, Faculty of Biology and Medicine, University of Oviedo, Asturias, Spain

Correspondence to: Dr. Jorge Tolivia, Departamento Morfología y Biología Celular, Facultad de Biología y Medicina, Universidad de Oviedo, Julián Clavería s/n, Oviedo 33006, Spain. E-mail: jtolivia{at}correo.uniovi.es

Apolipoprotein D (Apo D) is a secreted lipocalin in the nervous system that may be related to processes of reinnervation and regeneration. Under normal conditions, Apo D is present in the central nervous system in oligodendrocytes, astrocytes, and some scattered neurons. To elucidate the regional and cellular distribution of Apo D in normal human brain, we performed double immunohistochemistry for glial fibrillary acidic protein (GFAP) and Apo D in samples of postmortem human cerebral and cerebellar cortices. Most of the GFAP-positive cells in the gray matter had features of protoplasmic astrocytes and were mainly Apo D-positive. Apo D staining was mostly confined to the cell soma and proximal processes, whereas GFAP extended to a rich and extensive array of processes. The fibrous astrocytes in the white matter were immunoreactive for GFAP but not for Apo D. In the white matter, Apo D was mainly detected in oligodendrocytes and extracellularly in the neuropil. The results of the present study support a specific behavior for each astrocyte type. These findings suggest that Apo D expression may be cell-specific, depending on the particular tissue physiology at the time of examination.

(J Histochem Cytochem 52:1031–1036, 2004)

Key Words: apolipoprotein D • GFAP • astrocytes • protoplasmic • fibrous • human


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