Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
DOI: 10.1369/jhc.3A6243.2004
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kimoto, M.
Right arrow Articles by Ogawa, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kimoto, M.
Right arrow Articles by Ogawa, Y.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry
Volume 52 (8): 1057-1062, 2004
Copyright ©The Histochemical Society, Inc.

Carbonic Anhydrase VI in the Mouse Nasal Gland

Masaya Kimoto, Soichi Iwai, Takashi Maeda, Yoshiaki Yura, Ross T. Fernley and Yuzo Ogawa

Departments of Oral Pathology (MK,YO), Oral and Maxillofacial Surgery II (MK,SI,YY), and Oral Radiology (TM), Osaka University Graduate School of Dentistry, Osaka, Japan, and CSIRO Health Science and Nutrition, Victoria, Australia (RTF)

Correspondence to: Yuzo Ogawa, DDS, PhD, Dept. of Oral Pathology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan. E-mail: ogawa{at}dent.osaka-u.ac.jp

Western blotting analysis of mouse nasal tissue using a specific anti-mouse secreted carbonic anhydrase (CA VI) antibody has shown that CA VI is present in this tissue. A single immunoreactive band of 42 kD was observed, as has been found previously for salivary tissues. RT-PCR analysis has shown that nasal mucosa expressed CA VI mRNA. By immunohistochemistry (IHC), CA VI was observed in acinar cells, in duct contents of the anterior gland of the nasal septum, and in the lateral nasal gland. The Bowman's gland, the posterior gland of the nasal septum, and the maxillary sinus gland were negative. Immunoreactivity was also observed in the mucus covering the respiratory and olfactory mucosa and in the lumen of the nasolacrimal duct. In contrast, an anti-rat CA II antibody (that crossreacts with the mouse enzyme) stained only known CA II-positive cells and an occasional olfactory receptor neuron. These results indicate that CA VI is produced by the nasal gland and is secreted over the nasal mucosa. By reversible hydration of CO2, CA VI is presumed to play a role in mucosal functions such as CO2 sensation and acid–base balance. It may also play a role in olfactory function as a growth factor in maturation of the olfactory epithelial cells.

(J Histochem Cytochem 52:1057–1062, 2004)

Key Words: carbonic anhydrase VI • nasal gland • mouse • immunohistochemistry • Western analysis • RT-PCR


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
J. Hu, C. Zhong, C. Ding, Q. Chi, A. Walz, P. Mombaerts, H. Matsunami, and M. Luo
Detection of Near-Atmospheric Concentrations of CO2 by an Olfactory Subsystem in the Mouse
Science, August 17, 2007; 317(5840): 953 - 957.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
K. E. Ferris, R. D. Clark, and E. L. Coates
Topical Inhibition of Nasal Carbonic Anhydrase Affects the CO2 Detection Threshold in Rats
Chem Senses, March 1, 2007; 32(3): 263 - 271.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
A. Agrawal, S. Rengarajan, K. B. Adler, A. Ram, B. Ghosh, M. Fahim, and B. F. Dickey
Inhibition of mucin secretion with MARCKS-related peptide improves airway obstruction in a mouse model of asthma
J Appl Physiol, January 1, 2007; 102(1): 399 - 405.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2004