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DOI: 10.1369/jhc.3A6232.2004
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Journal of Histochemistry and Cytochemistry
Volume 52 (9): 1151-1157, 2004
Copyright ©The Histochemical Society, Inc.

Immunohistochemical Characterization of Cells in Adult Human Patellar Tendons

Fu Sai Chuen, Cheuk Yau Chuk, Wong Yim Ping, Wong Wan Nar, Hung Leung Kim and Chan Kai Ming

Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Shatin, NT, Hong Kong

Correspondence to: Chan Kai Ming, Dept. of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong. E-mail: kaimingchan{at}cuhk.edu.hk

Cells in tendons are conventionally identified as elongated tenocytes and ovoid tenoblasts, but specific markers for these cells are not available. The roles and interplay of these cells in tendon growth, remodeling, and healing are not well established. Therefore, we proposed to characterize these cells with respect to cell turnover, extracellular matrix metabolism, and expression of growth factors. Here we examined 14 healthy human patellar tendon samples for the expression of various proteins in tenocytes and tenoblasts, which were identified as elongated tendon cells and ovoid tendon cells, respectively. Matrix metalloproteinase 1 (MMP1), procollagen type I (procol I), heat shock protein 47 (hsp47), bone morphogenetic protein 12 (BMP12), 13 (BMP13), and transforming growth factor ß1 (TGFß1) were detected by immunohistochemistry (IHC). An image analysis of the IHC staining for proliferation cell nuclear antigen (PCNA) and apoptotic cells was performed to determine the proliferation index and the apoptosis index in elongated and ovoid tendon cells. The ovoid tendon cells expressed higher levels of procol I, hsp47, MMP1, BMP12, BMP13, and TGFß1 than the elongated tendon cells. Both the proliferation index and the apoptosis index of ovoid tendon cells were higher than those of the elongated tendon cells. The results suggested that ovoid tendon cells, conventionally recognized as tenoblasts, were more active in matrix remodeling. The expression of BMP 12, BMP13 and TGFß1 might be associated with the different cellular activities of tenoblasts and tenocytes. (J Histochem Cytochem 52:1151–1157, 2004)

Key Words: tenoblasts • tenocytes • MMP1 • procollagen type 1 • PCNA


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