Search:        >> Advanced Search
Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Martinovic, S. Articles by Vukicevic, S. Search for Related Content PubMed PubMed Citation Articles by Martinovic, S. Articles by Vukicevic, S. Social Bookmarking                      What's this?

Expression of Bone Morphogenetic Proteins in Stromal Cells from Human Bone Marrow Long-term Culture

Snjezana Martinovic, Sanja Mazic, Veronika Kisic, Nikolina Basic, Jasminka Jakic-Razumovic, Fran Borovecki, Drago Batinic, Petra Simic, Lovorka Grgurevic, Boris Labar and Slobodan Vukicevic

Department of Anatomy, Medical School University of Zagreb (SM,VK,FB,PS,LG,SV); Clinical Institute for Laboratory Diagnosis, Clinical Hospital Center Zagreb (SM,DB); and Departments of Internal Medicine (NB,BL) and Pathology (JJ-R), Clinical Hospital Center Zagreb, Zagreb, Croatia

Correspondence to: Slobodan Vukicevic, MD, PhD, Dept. of Anatomy, Medical School University of Zagreb, Salata 11, Croatia. E-mail: vukicev{at}mef.hr

Highly purified primitive hemopoietic stem cells express BMP receptors but do not synthesize bone morphogenetic proteins (BMPs). However, exogenously added BMPs regulate their proliferation, differentiation, and survival. To further explore the mechanism by which BMPs might be involved in hemopoietic differentiation, we tested whether stromal cells from long-term culture (LTC) of normal human bone marrow produce BMPs, BMP receptors, and SMAD signaling molecules. Stromal cells were immunohistochemically characterized by the presence of lyzozyme, CD 31, factor VIII, CD 68, S100, alkaline phosphatase, and vimentin. Gene expression was analyzed by RT-PCR and the presence of BMP protein was confirmed by immunohistochemistry (IHC). The supportive role of the stromal cell layer in hemopoiesis in vitro was confirmed by a colony assay of clonogenic progenitors. Bone marrow stromal cells express mRNA and protein for BMP-3, -4, and -7 but not for BMP-2, -5, and -6 from the first to the eighth week of culture. Furthermore, stromal cells express the BMP type I receptors, activin-like kinase-3 (ALK-3), ALK-6, and the downstream transducers SMAD-1, -4, and -5. Thus, human bone marrow stromal cells synthesize BMPs, which might exert their effects on hemopoietic stem cells in a paracrine manner through specific BMP receptors. (J Histochem Cytochem 52:1159–1167, 2004)

Key Words: BMPs • bone marrow stromal cells • hemopoiesis • long-term culture

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Jeanpierre, F. E. Nicolini, B. Kaniewski, C. Dumontet, R. Rimokh, A. Puisieux, and V. Maguer-Satta BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling Blood, October 15, 2008; 112(8): 3154 - 3163. [Abstract] [Full Text] [PDF]

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2004