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DOI: 10.1369/jhc.4A6350.2005
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Journal of Histochemistry and Cytochemistry
Volume 53 (1): 45-53, 2005
Copyright ©The Histochemical Society, Inc.

Upregulation of Neuronal Nitric Oxide Synthase mRNA and Protein in Adrenal Medulla of Water-deprived Rats

Feng-Jie Lai, Shiue-Shin Huang, Ming-Chia Hsieh, Shih-Chie Hsin, Chin-Han Wu, Ya-Chieh Hsin and Shyi-Jang Shin

Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan (FJL,SSH,MCH,SCH,CHW,SJS); Department of Dermatology, National Cheng-Kung University Hospital, Tainan, Taiwan (FJL); Division of Endocrinology and Metabolism, Kaohsiung Medical University, Kaohsiung, Taiwan (MCH,SCH,SJS); and University of Chicago, Chicago, Illinois (YCH)

Correspondence to: Shyi-Jang Shin, M.D., Ph.D., Division of Endocrinology and Metabolism, Kaohsiung Medical University, 100 Shih-Chuan First Road, Kaohsiung 80708, Taiwan. E-mail: sjshin{at}cc.kmu.edu.tw

Experiments were performed to investigate whether adrenal neuronal nitric oxide synthase (nNOS) mRNA and protein expression are responsive to alterations in body volume. Using an RT-PCR technique, the relative quantities of nNOS mRNA as well as the tyrosine hydroxylase and phenylethanolamine N-methyltransferase mRNA in the adrenals of water-deprived rats significantly increased from 12 hr to 4 days. In situ hybridization and immunohistochemical study showed that water deprivation activated nNOS mRNA and protein expression in the adrenal medulla. Four days after water deprivation, nNOS protein expression determined by Western blot significantly increased in the adrenal gland. Our results are the first to demonstrate that nNOS syntheses in the adrenal medulla are markedly increased in water-deprived rats. This study also indicates that the upregulation of nNOS synthesis of the adrenal medulla is associated with the activation of adrenal medullary function in the face of volume depletion. (J Histochem Cytochem 53:45–53, 2005)

Key Words: adrenal gland • neuronal nitric oxide synthase • water deprivation • rats


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