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Ultrastructural Studies and Na⁺,K⁺-ATPase Immunolocalization in the Antennal Urinary Glands of the Lobster Homarus gammarus (Crustacea, Decapoda)

Saber Khodabandeh, Guy Charmantier and Mireille Charmantier-Daures

Equipe Adaptation Ecophysiologique et Ontogenèse, Université Montpellier II, Montpellier, France (SK,GC,MC-D), and Faculty of Marine Sciences, University of Tarbiat Modarres, Tehran, Iran (SK)

Correspondence to: Mireille Charmantier-Daures, Equipe Adaptation Ecophysiologique et Ontogenèse, UMR 5171 GPIA, Université Montpellier II, cc 092, 34095 Montpellier cedex 05, France. E-mail: charmantier.daures{at}univ-montp2.fr

Unlike in crustacean freshwater species, the structure and ultrastructure of the excretory antennal gland is poorly documented in marine species. The general organization and ultrastructure of the cells and the localization of Na⁺,K⁺-ATPase were examined in the antennal gland of the adult lobster Homarus gammarus. Each gland is composed of a centrally located coelomosac surrounded ventrally by a labyrinth divided into two parts (I and II) and dorsally by a voluminous bladder. There is no differentiated nephridal tubule between them. The labyrinth and bladder cells have in common a number of ultrastructural cytological features, including basal membrane infoldings associated with mitochondria, apical microvilli, and cytoplasmic extrusions, and a cytoplasm packed with numerous vacuoles, vesicles, lysosome-like bodies, and swollen mitochondria. Each type of cell also presents distinctive characters. Na⁺,K⁺-ATPase was detected through immunofluorescence in the basal part of the cells of the labyrinth and in the bladder cells with an increasing immunostaining from labyrinth I to the bladder. No immunoreactivity was detected in the coelomosac. The cells of the labyrinth and of the bladder present morphological and enzymatic features of ionocytes. The antennal glands of the lobster thus possess active ion exchanges capabilities. (J Histochem Cytochem 53:1203–1214, 2005)

Key Words: excretory organ • osmoregulation • fine structure • immunolocalization • Na⁺,K⁺-ATPase

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