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Localization of P2X and P2Y Receptors in Dorsal Root Ganglia of the Cat

Huai-Zhen Ruan, Lori A. Birder, William C. de Groat, Changfeng Tai, James Roppolo, Charles A. Buffington and Geoffrey Burnstock

Autonomic Neuroscience Centre, Royal Free & University College Medical School, London, United Kingdom (H-ZR,GB); Department of Neurobiology, Third Military Medical University, Chongqing, China (H-ZR); University of Pittsburgh School of Medicine, Departments of Medicine (LAB) and Pharmacology (LAB,WCdG,CT,JR), Pittsburgh, Pennsylvania; and Ohio State University Department of Clinical Veterinary Sciences, Columbus Ohio (CAB)

Correspondence to: Prof. Geoffrey Burnstock, Autonomic Neuroscience Centre, Royal Free & University College Medical School, Rowland Hill Street, London NW3 2PF, United Kingdom. E-mail: g.burnstock{at}ucl.ac.uk

The distribution of P2X and P2Y receptor subtypes in upper lumbosacral cat dorsal root ganglia (DRG) has been investigated using immunohistochemistry. Intensity of immunoreactivity for six P2X receptors (P2X₅ receptors were immuno-negative) and the three P2Y receptors examined in cat DRG was in the order of P2Y₂ = P2Y₄>P2X₃>P2X₂ = P2X₇>P2X₆>P2X₁ = P2X₄>P2Y₁. P2X₃, P2Y₂, and P2Y₄ receptor polyclonal antibodies stained 33.8%, 35.3%, and 47.6% of DRG neurons, respectively. Most P2Y₂, P2X₁, P2X₃, P2X₄, and P2X₆ receptor staining was detected in small- and medium-diameter neurons. However, P2Y₄, P2X₂, and P2X₇ staining was present in large- and small-diameter neurons. Double-labeling immunohistochemistry showed that 90.8%, 32.1%, and 2.4% of P2X₃ receptor-positive neurons coexpressed IB₄, CGRP, and NF200, respectively; whereas 67.4%, 41.3%, and 39.1% of P2Y₄ receptor-positive neurons coexpressed IB₄, CGRP, and NF200, respectively. A total of 18.8%, 16.6%, and 63.5% of P2Y₂ receptor-positive neurons also stained for IB₄, CGRP, and NF200, respectively. Only 30% of DRG neurons in cat were P2X₃-immunoreactive compared with 90% in rat and in mouse. A further difference was the low expression of P2Y₁ receptors in cat DRG neurons compared with more than 80% of the neurons in rat. Many small-diameter neurons were NF200-positive in cat, again differing from rat and mouse. (J Histochem Cytochem 53:1273–1282, 2005)

Key Words: purinoceptors • P2X receptor • P2Y receptor • ATP • dorsal root ganglion • cat

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