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Differential Expression of Laminin Isoforms in Ovarian Epithelial Carcinomas Suggesting Different Origin and Providing Tools for Differential Diagnosis

Marko Määttä, Ralf Bützow, Jani Luostarinen, Noora Petäjäniemi, Taina Pihlajaniemi, Sirpa Salo, Kaoru Miyazaki, Helena Autio-Harmainen and Ismo Virtanen

Department of Pathology (MM,HA-H), Collagen Research Unit, Biocenter Oulu, Department of Medical Biochemistry and Molecular Biology (JL,TP), and Department of Biochemistry (SS), University of Oulu, Oulu, Finland; Department of Pathology, HUSLAB, Haartman Institute and Department of Obstetrics and Gynecology, Research Laboratory, Biomedicum, Helsinki University Central Hospital, Finland (RB); Institute of Biomedicine/Anatomy, University of Helsinki, Helsinki, Finland (NP,IV); and Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, Maioka-cho, Totsuka-ku, Yokohama, Japan (KM)

Correspondence to: Marko Määttä, MD, PhD, Department of Ophthalmology, University of Helsinki, PO Box 220, 00029 HUS Helsinki, Finland. E-mail: mmaatta{at}mailcity.com

Immunohistochemistry was used to study the distribution of laminin (Ln) chains, collagen types IV ( 1/2), VII, and XVIII and Lutheran antigen (Lu) in 36 frozen ovarian carcinoma samples. Surface epithelial basement membrane (BM) of the normal ovary showed immunoreactivity for Ln 1, 3-5, ß1-3, 1, and 2 chains and type IV and XVIII collagens. Chains of Ln-5 (3ß32) and Ln-10 (5ß11) as well as type IV and XVIII collagens were found in most tumor BMs, but Ln 2 chain and type VII collagen were detected only in few tumors. Contrary to serous tumors, BMs of mucinous carcinomas showed Ln 4 chain, but not Ln 1 and ß2 chains. Ln 1 chain was found in most endometrioid carcinomas, whereas chains of Ln-5 were only moderately detectable in comparison with serous and mucinous carcinomas. In the normal ovary, Lu immunoreactivity was confined to basal aspect in the ovarian epithelial cells, but in tumor specimens Lu immunostainings showed variable polarized and nonpolarized patterns. The results suggest that the three types of ovarian carcinoma have distinct differences in their Ln distribution and can be grouped based on their expression pattern. This suggests that they may have histogenetically different precursors and may help to distinguish these tumors from each other.

(J Histochem Cytochem 53:1293–1300, 2005)

Key Words: basement membrane • carcinoma • collagen • invasion • laminin • Lutheran • ovary

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