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Mucin Granules are in Close Contact with Tubular Elements of the Endoplasmic Reticulum

Juan Perez-Vilar, Carla M. Pedrosa Ribeiro, Wendy C. Salmon, Raean Mabolo and Richard C. Boucher

Cystic Fibrosis/Pulmonary Research and Treatment Center, School of Medicine (JP-V,CMPR,RM,RCB), Michael Hooker Microscopy Facility (WCS), University of North Carolina, Chapel Hill, North Carolina

Correspondence to: Juan Perez-Vilar, Cystic Fibrosis/Pulmonary Research and Treatment Center, School of Medicine, CB#7248, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7248. E-mail: juan_vilar{at}med.unc.edu

Live cell imaging methods were used to characterize goblet cells expressing a MUC5AC domain fused to enhanced green fluorescent protein that labels the granule lumen. Golgi complex and endosome/lysosome elements largely resided in the periphery of the granular mass. On the contrary, a tubular meshwork of endoplasmic reticulum (ER) was in close contact with the mucin granules. This meshwork could be identified in fixed native human bronchial goblet cells labeled with an anti-calreticulin antibody. The potential biological significance of this ER network for mucin secretion is discussed.

(J Histochem Cytochem 53:1305–1309, 2005)

Key Words: goblet cell • mucous cell • mucin granule • endoplasmic reticulum • mucins • organelles • FRAP

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