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Expression of Versican and ADAMTS1, 4, and 5 During Bone Development in the Rat Mandible and Hind Limb

Megumi Nakamura, Shinya Sone, Ichiro Takahashi, Itaru Mizoguchi, Seishi Echigo and Yasuyuki Sasano

Divisions of Oral Surgery (MN,SE), Craniofacial Development and Regeneration (MN,YS), Pediatric Dentistry (SS), and Orthodontics and Dentofacial Orthopedics (IT), Tohoku University Graduate School of Dentistry, Sendai, Japan, and Department of Orthodontics, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, Japan (IM)

Correspondence to: Yasuyuki Sasano, DDS, PhD, Division of Craniofacial Development and Regeneration, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan. E-mail: sasano{at}anat.dent.tohoku.ac.jp

Extracellular matrix (ECM) remodeling is achieved by both production and degradation of ECM molecules during bone development. ADAMTS (a disintegrin and metalloprotease with thrombospondin type 1 motifs) constitutes a family of extracellular proteases which are implicated in cleaving the protein versican. The present study was designed to investigate the expression of versican and ADAMTS1, 4, and 5 mRNA during bone development in rat mandibles and hind limbs by RT-PCR and in situ hybridization. Versican was localized by immunohistochemistry. The process of bone development from day 14 postcoitum through week 6 postnatum was divided into the beginning of osteogenesis, woven bone, and lamellar bone stages. Versican protein was abundant in the woven bone matrix, but decreased in the lamellar bone matrix. Versican mRNA was prominent in some osteoblasts with corresponding localization of the cognate protein. The temporal and spatial mRNA expression pattern of ADAMTS1, 4, and 5 was comparable to that of versican. These results suggest that woven bone rich in versican alters into lamellar bone containing little versican during bone development in both mandibles and hind limbs, where some osteoblasts may be involved in production as well as degradation of versican by secreting ADAMTS1, 4, and 5. (J Histochem Cytochem 53:1553–1562, 2005)

Key Words: versican • ADAMTS • bone • development • osteoblast • extracellular matrix • remodeling • in situ hybridization • immunohistochemistry • RT-PCR

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