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DOI: 10.1369/jhc.4A6457.2005
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Journal of Histochemistry and Cytochemistry
Volume 53 (2): 177-185, 2005
Copyright ©The Histochemical Society, Inc.

Histomathematical Analysis of Clinical Specimens : Challenges and Progress

Gallya Gannot, John W. Gillespie, Rodrigo F. Chuaqui, Michael A. Tangrea, W. Marston Linehan and Michael R. Emmert-Buck

Pathogenetics Unit, Laboratory of Pathology and Urologic Oncology Branch (GG,JWG,RFC,MAT,MRE-B), and Urologic Oncology Branch (WML), Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland

Correspondence to: Michael R. Emmert-Buck, Pathogenetics Unit, Advanced Technology Center, Laboratory of Pathology and Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, 8717 Grovemont Circle, Bethesda, MD 20892-4605. E-mail: mbuck{at}helix.nih.gov

Proteomic analysis of clinical tissue specimens is a difficult undertaking. Described here is a multiplex study of protein expression levels in histological sections of human prostate that addresses many of the associated challenges. Whole-mount sections from 10 prostatectomy specimens were studied using 15 antibodies, immunohistochemical staining, digital imaging, and mathematical analysis of the data sets. The approach was successful in stratifying cell lineages present in the samples based on proteomic patterns, including differentiating normal epithelium from cancer. This strategy likely will be a useful method for extending the number of proteins that can be analyzed in clinical cancer specimens using currently available laboratory techniques.

(J Histochem Cytochem 53:177–185, 2005)

Key Words: histomathematics • histopathology • mathematics • immunohistochemistry • prostate cancer


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