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DOI: 10.1369/jhc.4A6280.2005
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Journal of Histochemistry and Cytochemistry
Volume 53 (2): 207-216, 2005
Copyright ©The Histochemical Society, Inc.

Behavior of Transplanted Bone Marrow–derived GFP Mesenchymal Cells in Osteochondral Defect as a Simulation of Autologous Transplantation

Yasushi Oshima, Nobuyoshi Watanabe, Ken-ichi Matsuda, Shinro Takai, Mitsuhiro Kawata and Toshikazu Kubo

Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan (YO,NW,TK), Department of Anatomy and Neurobiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan (KIM,MK), and Department of Orthopaedic Surgery, Teikyo University School of Medicine, Tokyo, Japan (ST)

Correspondence to: Yasushi Oshima, MD, Connective Tissue Biochemistry, Department of Orthopaedics, University of California San Diego, 9500 Gilman Dr. MTF Bldg. Rm. 134, La Jolla, CA 92093-0630. E- mail: yasushi{at}sj8.so-net.ne.jp

To elucidate the behavior of autologously transplanted mesenchymal cells in osteochondral defects, we followed transplanted cells using green fluorescent protein (GFP) transgenic rats, in which all cells express GFP signals in their cytoplasm and nuclei as transplantation donors. Bone marrow–derived mesenchymal cells, which contain mesenchymal stem cells (MSCs), were obtained from transgenic rats. Then, dense mesenchymal cell masses created by hanging-drop culture were transplanted and fixed with fibrin glue into osteochondral defects of wild-type rats. At 24 weeks after surgery, the defects were repaired with hyaline-like cartilage and subchondral bone. GFP positive cells, indicating transplanted mesenchymal-derived cells, were observed in the regenerated tissues for 24 weeks although GFP positive cells decreased in number with time. Because GFP causes no immunological rejection and requires no chemicals for visualization, transplantation between transgenic and wild-type rats can be regarded as a simulation of autologous transplantation, and the survivability of transplanted cells are able to be followed easily and reliably. Thus, the behavior of transplanted mesenchymal cells was able to be elucidated in vivo by this strategy, and the results could be essential in future tissue engineering for the regeneration of osteochondral defects with original hyaline cartilage and subchondral bone. (J Histochem Cytochem 53:207–216, 2005)

Key Words: osteochondral defect • mesenchymal cells • green fluorescent protein • transgenic animal


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