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DOI: 10.1369/jhc.4B6507.2005
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Journal of Histochemistry and Cytochemistry
Volume 53 (2): 243-247, 2005
Copyright ©The Histochemical Society, Inc.

BRIEF REPORT

Localization of Sphingosine Kinase-1 in Mouse Sperm Acrosomes

Kenji Matsumoto, Yoshiko Banno, Takashi Murate, Yukihiro Akao and Yoshinori Nozawa

Gifu International Institute of Biotechnology, Gifu, Japan (KM,YA,YN); Department of Biochemistry, Gifu University School of Medicine, Gifu, Japan (YB); and Nagoya University School of Health Science, Nagoya, Japan (TM)

Correspondence to: Kenji Matsumoto, Ph.D., Gifu International Institute of Biotechnology, 1-1 Naka-Fudogaoka, Kakamigahara, Gifu 504-0838, Japan. E-mail: kmatsumo{at}giib.or.jp

Sphingosine kinase (SPHK) catalyzes sphingosine phosphorylation to form a bioactive lipid mediator, sphingosine-1-phosphate (S1P). In the current study, we report the presence of SPHK-1 in mouse spermatozoa. SPHK-1 was localized to the acrosomes of spermatozoa, and its expression was proven by RT-PCR and Western blot analysis. SPHK activity of mouse spermatozoa was 18.1 pmol/min/mg protein. Furthermore, we identified the presence of the S1P receptors S1P1, S1P2, S1P3, and S1P5, in mouse spermatozoa by RT-PCR. These results suggest that S1P produced by SPHK-1 would play a role in the acrosomal reaction through S1P receptors. (J Histochem Cytochem 53:243–247, 2005)

Key Words: sphingosine kinase-1 • sphingosine-1-phosphate • sperm • acrosome


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