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DOI: 10.1369/jhc.3B6194.2005
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Journal of Histochemistry and Cytochemistry
Volume 53 (2): 249-252, 2005
Copyright ©The Histochemical Society, Inc.

BRIEF REPORT

Introduction of Tyramide Signal Amplification (TSA) to Pre-embedding Nanogold–Silver Staining at the Electron Microscopic Level

Seung-won Lee, Song Eun Lee, Seong Hyuk Ko, Eun Kyoung Hong, Kwang Il Nam, Kei-ichiro Nakamura, Shuhei Imayama, Yeong-Joon Park, Kyu Youn Ahn, Choon Sang Bae, Baik Yoon Kim and Sung Sik Park

Department of Anatomy, Chonnam National University Medical School, Gwangju, Korea (S-wL,SEL,SHK,EKH, KIN,KYA,CSB,BYK,SSP); Chonnam National University Dental Materials Research Institute, Gwangju, Korea (Y-JP); Department of Anatomy and Histology, Kurume University School of Medicine, Kurume, Japan (K-iN); and Department of Dermatology, National Kyushu Medical Center, Fukuoka, Japan (SI)

Correspondence to: Dr. Seung-won Lee, Dept. of Anatomy, Chonnam National University Medical School, Gwangju 501-746, Korea. E-mail: seunglee{at}chonnam.ac.kr

The tyramide signal amplification (TSA) technique has been shown to detect scarce tissue antigens in light and electron microscopy. In this study we applied the TSA technique at the electron microscopic level to pre-embedding immunocytochemistry. This protocol was compared to the non-amplified protocol. With the TSA protocol, the labeling of GM130, a cis-Golgi matrix protein, was tested in a cell line and found to be highly sensitive and more enhanced than that with the simple protocol. Moreover, the gold particles were well localized to the cis-side of the Golgi apparatus in both the TSA and the simple protocol. (J Histochem Cytochem 53:249–252, 2005)

Key Words: tyramide signal amplification • pre-embedding • immunocytochemistry


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